Transmitter content and afferent connections of estrogen-sensitive progestin receptor-containing neurons in the primate hypothalamus.
Transmitter content and afferent connections of estrogen-sensitive progestin receptor-containing neurons in the primate hypothalamus.
复制标题
灵长类动物下丘脑中含有雌激素敏感孕激素受体的神经元的递质含量和传入连接。
DOI：
10.1159/000126187
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发表时间：
1992
影响因子：
4.1
通讯作者：
Naftolin,F
中科院分区：
文献类型：
--
作者：
Leranth,C;MacLusky,NJ;Brown,TJ;Chen,EC;RedmondJr,DE;Naftolin,F
用免疫细胞化学双标记方法，对成年绿色猕猴（Cercopithecus aethiops）下丘脑含孕激素受体细胞的解剖位置、神经递质含量和传入联系进行了研究。切除动物卵巢并给予戊酸雌二醇或油注射溶剂，并在给药10天后处死。使用单克隆抗体提出对兔子宫卵泡素受体（PR），PR免疫反应细胞的分布在下丘脑内侧基底部和雌激素治疗对这种分布的影响进行了测定。PR免疫反应细胞被发现在整个腹内侧核（VMN），在VMN和穹窿之间的区域，并在漏斗核的内侧部分。雌激素处理显着增加标记细胞的数量和免疫反应产物的强度在这些地区。在双重免疫染色切片中，证明了对5-羟色胺、阿黑皮素原衍生肽、GABA、儿茶酚胺、神经肽Y、P物质、胆囊收缩素和生长抑素的抗原呈免疫反应的终扣与PR免疫反应的下丘脑神经元的索马体建立突触接触。在秋水仙碱预处理的动物，所有PR-下丘脑中的神经元被发现含有谷氨酸脱羧酶，GABA的合成所需的酶的免疫反应性。未观察到与其他抗原（包括LHRH）共定位的证据。因为已知LHRH神经元接受丰富的GABA能神经支配，所以含PR的GABA能细胞可能代表类固醇敏感位点，用于整合来自参与促性腺激素分泌控制的其他神经系统的输入。
Progestin receptor-containing cells in the hypothalamus of the adult female green monkey (Cercopithecus aethiops) were examined by double-label immunocytochemical methods to determine their anatomical location, neurotrans-mitter content and afferent connections. Animals were ovariectomized and administered either estradiol valerate or the oil injection vehicle, and were sacrificed after 10 days of treatment. Using a monoclonal antibody raised against rabbit uterine progestin receptor (PR), the distribution of PR-immunoreactive cells in the mediobasal hypothalamus and the effect of estrogen treatment on this distribution was determined. PR-immunoreactive cells were found throughout the ventromedial nucleus (VMN), in the area between the VMN and fornix, and in the medial portion of the infundibular nucleus. Estrogen treatment dramatically increased both the number of labeled cells and the intensity of immunoreaction product in these regions. In double-immunostained sections, boutons immunoreactive for antigens indicative of serotonin, pro-opiomelanocortin derived peptides, GABA, catecholamine, neuropeptide Y, substance P, cholecystokinin, and somatostatin were demonstrated to establish synaptic contact with the soma of PR-immunoreactive hypotha-lamic neurons. In colchicine-pretreated animals, all PR-containing neurons in the mediobasal hypothalamus were found to contain immunoreactivity for glutamic acid decarboxylase, the enzyme required for synthesis of GABA. No evidence of colocalization with other antigens, including LHRH, was observed. Because LHRH neurons are known to receive a rich GABAergic innervation PR-containing GABAergic cells may represent steroid-sensitive sites of integration for inputs from other neural systems involved in the control of gonadotropin secretion.