Modulation of lyso-platelet-activating factor: acetyl-CoA acetyltransferase from rat splenic microsomes. The role of calcium ions.
Modulation of lyso-platelet-activating factor: acetyl-CoA acetyltransferase from rat splenic microsomes. The role of calcium ions.
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溶血血小板激活因子的调节：来自大鼠脾微粒体的乙酰辅酶A乙酰转移酶。
DOI：
10.1016/0167-4889(85)90218-6
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
M. Sánchez
中科院分区：
文献类型：
--
作者：
J. Gomez;M. Nieto;J. Mato;M. Sánchez
酶溶血血小板活化因子：乙酰辅酶A乙酰转移酶（EC 2.3。1.67）在大鼠脾脏微粒体组分中进行测定。加入微摩尔Ca 2+可迅速增强乙酰转移酶活性，而加入过量Ca 2+的EGTA可逆转这种激活作用。Ca 2+对乙酰辅酶A的表观Km有影响，对乙酰化反应的Vmax无明显影响。当在5 mM EGTA存在下分离微粒体以去除内源性钙调素时，观察到Ca 2+对乙酰化反应的相同增强作用。添加外源性钙调素对酶活性没有影响。预孵育的脾微粒体与钙调蛋白抑制剂三氟拉嗪降低乙酰转移酶的存在和不存在的Ca 2+，表明这种药物的影响独立的钙调蛋白。向测定混合物中加入Mg-ATP也对乙酰化反应没有影响。这些数据表明，Ca 2+调节乙酰转移酶活性从大鼠脾微粒体的机制，似乎是独立的钙调素或蛋白磷酸化。
The enzyme lyso-platelet-activating factor: acetyl-CoA acetyltransferase (EC 2.3. 1.67) was assayed in microsomal fractions from rat spleens. The addition of micromolar Ca 2+ rapidly enhanced acetyltransferase activity and this activation was reversed by the addition of EGTA in excess of Ca 2+. The effect of Ca 2+ was on the apparent K m of the enzyme for the substrate acetyl-CoA without showing any significant effect on the V max of the acetylation reaction. When microsomes were isolated in the presence of 5 mM EGTA, to remove endogenous calmodulin, the same enhancing effect of Ca 2+ on the acetylation reaction was observed. The addition of exogenous calmodulin to this preparation had no effect on the enzyme activity. Preincubation of spleen microsomes with the calmodulin inhibitor trifluoperazine decreased acetyltransferase in both the presence and the absence of Ca 2+, indicating an effect of this drug independently of calmodulin. The addition of Mg-ATP to the assay mixture also had no effect on the acetylation reaction. These data suggest that Ca 2+ modulates acetyltransferase activity from rat spleen microsomes by a mechanism that seems to be independent of calmodulin or protein phosphorylation.
大鼠肺泡巨噬细胞从 1-烷基-2-酰基-sn-甘油-3-磷酸胆碱生物合成 1-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱（血小板激活因子）。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Albert,DH;Snyder,F
通讯作者：
Snyder,F
中性粒细胞质膜囊泡中 ATP 依赖性钙转运。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ochs,DL;Reed,PW
通讯作者：
Reed,PW
血小板激活因子合成的调节：通过大鼠脾微粒体中的磷酸化和去磷酸化调节1-烷基-2-溶血-sn-甘油-3-磷酸胆碱：乙酰辅酶A乙酰转移酶。
DOI：
10.1016/s0006-291x(84)80182-5
发表时间：
1984
影响因子：
3.1
作者：
Lenihan,DJ;Lee,TC
通讯作者：
Lee,TC