Immunolocalisation of crustacean-SIFamide in the median brain and eyestalk neuropils of the marbled crayfish
Immunolocalisation of crustacean-SIFamide in the median brain and eyestalk neuropils of the marbled crayfish
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DOI：
10.1007/s00441-007-0473-8
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发表时间：
2007-11-01
影响因子：
3.6
通讯作者：
Harzsch, S.
中科院分区：
文献类型：
--
作者：
Polanska, M. A.;Yasuda, A.;Harzsch, S.
甲壳动物-SIFamide（GYRKPPFNGSIFamide）是最近从螯虾神经组织中分离得到的一种新的神经肽。我们绘制了这种肽在大理石纹小龙虾（Marmorkrebs）（一种单性生殖甲壳动物）的大脑中部和眼柄神经乳头中的定位。我们的实验表明，甲壳类动物SIFamide强烈表达在所有主要车厢的小龙虾的大脑，包括所有三个视神经，外侧procerebrum与半椭圆体，和内侧procerebrum与中央复合体。这些发现意味着这种肽在视觉处理中的作用已经在椎板的水平上，但也在更深的中继站的水平上。免疫标记是特别强的副叶和中脑嗅叶，从第一个天线接收化学感觉输入。大多数细胞的嗅球束，投射神经元通路，连接中脑和前脑，被标记。这条通路在将触觉和嗅觉刺激传递到外侧前脑中起着核心作用，在外侧前脑中，这种输入与视觉信息会聚。弱标记也存在于与机械感觉第二触角相关的tritcerebrum中。两者合计，我们建议的甲壳类动物SIFamidergic神经元在处理高阶，多模态输入的小龙虾大脑中的重要作用。
Crustacean-SIFamide (GYRKPPFNGSIFamide) is a novel neuropeptide that was recently isolated from crayfish nervous tissue. We mapped the localisation of this peptide in the median brain and eyestalk neuropils of the marbled crayfish (Marmorkrebs), a parthenogenetic crustacean. Our experiments showed that crustacean-SIFamide is strongly expressed in all major compartments of the crayfish brain, including all three optic neuropils, the lateral protocerebrum with the hemiellipsoid body, and the medial protocerebrum with the central complex. These findings imply a role of this peptide in visual processing already at the level of the lamina but also at the level of the deeper relay stations. Immunolabelling is particularly strong in the accessory lobes and the deutocerebral olfactory lobes that receive a chemosensory input from the first antennae. Most cells of the olfactory globular tract, a projection neuron pathway that links deuto- and protocerebrum, are labelled. This pathway plays a central role in conveying tactile and olfactory stimuli to the lateral protocerebrum, where this input converges with optic information. Weak labelling is also present in the tritocerebrum that is associated with the mechanosensory second antennae. Taken together, we suggest an important role of crustacean-SIFamidergic neurons in processing high-order, multimodal input in the crayfish brain.