Ovarian cancer immuno-reactive antigen domain containing 1 (OCIAD1), a key player in ovarian cancer cell adhesion.
Ovarian cancer immuno-reactive antigen domain containing 1 (OCIAD1), a key player in ovarian cancer cell adhesion.
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卵巢癌免疫反应抗原结构域含有 1 (OCIAD1)，在卵巢癌细胞粘附中发挥关键作用。
DOI：
10.1016/j.ygyno.2007.12.024
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发表时间：
2008
影响因子：
4.7
通讯作者：
R. Ganapathi
中科院分区：
文献类型：
--
作者：
S. Sengupta;C. Michener;P. Escobar;J. Belinson;R. Ganapathi
CAPTIVESTo确定转移性卵巢癌独特的蛋白质，并测试其潜在的参与cell adhesion. METHODS我们纯化质膜从配对的转移和原发性肿瘤组织与IIIC期卵巢癌患者。通过液相色谱质谱法（LC-MS）鉴定转移瘤特有的膜蛋白。在LPA存在下，使用过表达和下调方法确定所鉴定的蛋白质之一，卵巢癌免疫反应性抗原结构域1（OCIAD 1）在细胞粘附中的作用。OCIAD 1在HEY卵巢癌细胞中的过表达增加了LPA诱导的细胞与细胞外基质蛋白胶原I和层粘连蛋白10/11的粘附，但没有增加基础水平的细胞粘附。LY 294002和GF 109203 X没有阻断这种增强作用，表明OCIAD 1不使用PKC和PI 3 K信号通路来发挥其对粘附的作用。此外，LPA诱导的细胞粘附胶原蛋白I不受紫杉醇（5 μM）在OCIAD 1过表达cells. CONCLUSION这是第一个报告，OCIAD 1是过度表达在转移性卵巢癌组织。OCIAD 1对细胞粘附的影响可能与其在卵巢癌中的功能有关。紫杉醇在OCIAD 1存在下不能影响卵巢癌细胞粘附，这增加了OCIAD 1在肿瘤转移中的作用的可能性。正在进行的使用小鼠原位LPA依赖性卵巢癌转移模型的研究集中在抑制OCIAD 1在肿瘤转移中的潜在作用的策略上。
OBJECTIVESTo identify proteins unique to metastatic ovarian cancer and test their potential involvement in cell adhesion.METHODSWe purified plasma membrane from paired metastatic and primary tumor tissues from patients with stage IIIC ovarian cancer. Membrane proteins unique to metastases were identified by liquid chromatographic mass spectrometry (LC-MS). The role of one of the identified proteins, ovarian cancer immuno-reactive antigen domain containing 1 (OCIAD1) in cell adhesion was determined in the presence of LPA using both over-expression and down regulation approaches.RESULTSWe identified a differentially expressed 29 kDa protein as OCIAD1 over-expressed in metastatic tissues, when compared to primary tumor tissues. OCIAD1 over-expression in HEY ovarian cancer cells increased LPA-induced, but not basal level cell adhesion to extracellular matrix proteins collagen I and laminin 10/11. This enhancement was not blocked by LY294002 and GF109203X, suggesting that OCIAD1 does not use PKC and PI3K signaling pathways to exert its effect on adhesion. In addition, LPA induced cell adhesion to collagen I was unaffected by paclitaxel (5 µM) in OCIAD1 overexpressing cells.CONCLUSIONSThis is the first report that OCIAD1 is over-expressed in metastatic ovarian cancer tissues. The effect of OCIAD1 on cell adhesion may be related to its function in ovarian cancer. Failure of paclitaxel to affect ovarian cancer cell adhesion in presence of OCIAD1 raises the possibility of OCIAD1's role in tumor metastasis. Ongoing studies using a mouse orthotopic LPA-dependent ovarian cancer metastasis model are focused on strategies to inhibit the potential role of OCIAD1 in tumor metastasis.
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影响因子：
3.6
作者：
Baudhuin, LM;Cristina, KL;Xu, Y
通讯作者：
Xu, Y
DOI：
--
发表时间：
2000-03
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Limin Hu;C. Zaloudek;G. Mills;J. Gray;R. Jaffe
通讯作者：
Limin Hu;C. Zaloudek;G. Mills;J. Gray;R. Jaffe
一种与 α1-肾上腺素能受体偶联的新型鸟嘌呤核苷酸结合蛋白。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Im,MJ;Riek,RP;Graham,RM
通讯作者：
Graham,RM
影响因子：
11.2
作者：
S. Johnson;P. B. Laub;J. Beesley;R. Ozols;T. C. Hamilton
通讯作者：
S. Johnson;P. B. Laub;J. Beesley;R. Ozols;T. C. Hamilton