Improved procedures for purification of the Bandeiraea simplicifolia I isolectins and Bandeiraea simplicifolia II lectin by affinity chromatography.
Improved procedures for purification of the Bandeiraea simplicifolia I isolectins and Bandeiraea simplicifolia II lectin by affinity chromatography.
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改进了通过亲和层析纯化 Bandeiraea simplicifolia I 同种凝集素和 Bandeiraea simplicifolia II 凝集素的程序。
DOI：
10.1111/j.1432-1033.1980.tb07197.x
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Goldstein,IJ
中科院分区：
文献类型：
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作者：
Delmotte,FM;Goldstein,IJ
Bandeiraea simplicifolia植物种子含有五种α-d-半乳糖基结合凝集素家族（BS I-A4、A3 B、A2 B2、AB 3、B4）和N-乙酰基-d-葡萄糖胺结合凝集素（BS II）。经Pi/NaCl萃取和硫酸铵分级分离后，BS II特异性吸附在-氨基苄基-1-硫代-N-乙酰基-β-d-氨基葡萄糖苷-琥珀酰氨基己基氨基-Sepharose-4 B上。BS I异凝集素通过该柱，BS II通过含有2 mMN-乙酰基-d-葡糖胺的Pi/NaCl或0.1 M乙酸钠缓冲液（pH 3.6）选择性洗脱。将未与色谱柱结合的材料上样至顶部-氨基苯基-β-d-吡喃半乳糖基-琥珀酰氨基己基氨基-琼脂糖4 B上。BS I-A4在含有1 mMN-乙酰基-d-半乳糖胺的Pi/NaCl中以尖锐峰特异性洗脱。然后，BSI-A3 B、A2 B2、AB 3和B4分别用含有3 mM、8 mM、15 mM和50 mM甲基α-d-吡喃半乳糖苷的Pi/NaCl选择性洗脱，对于每种同种凝集素呈单峰。
Bandeiraea simplicifoliaplant seeds contain a family of five α‐d‐gal actopyranosyl‐binding isolectins (BS I‐A4, A3B, A2B2, AB3, B4) andN‐acetyl‐d‐glucosamine‐binding lectin (BS II). After Pi/NaCl extraction and ammonium sulfate fractionation BS II is specifically adsorbed ontop‐aninobenzyl‐1‐thio‐N‐acetyl‐β‐d‐glucosaminide‐succinylaminohexylaminyl—Sepharose‐4B. The BS I isolectins pass through this column and BS II is selectively eluted by Pi/NaCl containing 2 mMN‐acetyl‐d‐glucosamine or by 0.1 M sodium acetate buffer pH 3.6. The material not bound to the column is loaded ontop‐aminophenyl‐β‐d‐galactopyranosyl‐succinylaminohexylaminyl—Sepharose 4B. BS I‐A4is specifically eluted in a sharp peak with Pi/NaCl containing 1 mMN‐acetyl‐d‐galactosamine. Then, BS I‐A3B, A2B2, AB3and B4are selectively eluted, in a single peak for each isolectin, with Pi/NaCl containing 3 mM, 8 mM, 15 mM and 50 mM methyl α‐d‐galactopyranoside, respectively.