Purification, crystallization and preliminary X-ray analysis of two nudix hydrolases from Deinococcus radiodurans.
Purification, crystallization and preliminary X-ray analysis of two nudix hydrolases from Deinococcus radiodurans.
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来自耐辐射奇球菌的两种 nudix 水解酶的纯化、结晶和初步 X 射线分析。
DOI：
10.1107/s0907444903002671
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
S. Holbrook
中科院分区：
文献类型：
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作者：
E. L. Holbrook;U. Schulze;G. W. Buchko;S. Ni;M. Kennedy;S. Holbrook
两种来自耐辐射球菌的 nudix 水解酶已被纯化和结晶。 DR1025 和 DR0079 的衍射数据分辨率分别为 1.4 和 1.9 A。 DR1025属于空间群P4(1)2(1)2/P4(3)2(1)2，晶胞参数a = b = 53.2，c = 122.6 A（晶胞体积346 883 A(3)，V(M) = 2.5 A(3) Da(-1)，溶剂含量50.2%）。 DR0079属于空间群C222(1)，晶胞参数a = 34.1，b = 157.2，c = 126.5 A（晶胞体积677 308 A(3)，V(M) = 2.2 A(3) Da(-1)，溶剂含量44.0%）。计算出的 DR1025 细胞含量表明不对称单元中存在一个分子。动态光散射和凝胶过滤表明它是溶液中的二聚体。 DR0079 的空间群和晶胞参数表明每个不对称单元存在两个分子。凝胶过滤和核磁共振波谱表明它是溶液中的单体。
Two nudix hydrolases from Deinococcus radiodurans have been purified and crystallized. Diffraction data have been collected to 1.4 and 1.9 A resolution for DR1025 and DR0079, respectively. DR1025 belongs to space group P4(1)2(1)2/P4(3)2(1)2, with unit-cell parameters a = b = 53.2, c = 122.6 A (unit-cell Volume 346 883 A(3), V(M) = 2.5 A(3) Da(-1), solvent content 50.2%). DR0079 belongs to space group C222(1), with unit-cell parameters a = 34.1, b = 157.2, c = 126.5 A (unit-cell Volume 677 308 A(3), V(M) = 2.2 A(3) Da(-1), solvent content 44.0%). The calculated cell content of DR1025 indicates the presence of one molecule in the asymmetric unit. Dynamic light scattering and gel filtration suggest it to be a dimer in solution. The space group and unit-cell parameters of DR0079 indicate the presence of two molecules per asymmetric unit. Gel filtration and NMR spectroscopy suggest it to be a monomer in solution.