Aqueous extract of Yin-Chen-Hao decoction, a traditional Chinese prescription, exerts protective effects on concanavalin A-induced hepatitis in mice through inhibition of NF-κB
Aqueous extract of Yin-Chen-Hao decoction, a traditional Chinese prescription, exerts protective effects on concanavalin A-induced hepatitis in mice through inhibition of NF-κB
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DOI：
10.1211/jpp.58.5.0013
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发表时间：
2006-05-01
影响因子：
3.3
通讯作者：
Jin, Miao-Wen
中科院分区：
文献类型：
--
作者：
Cai, Hui;Song, Yao-Hong;Jin, Miao-Wen
在传统的东方医学中，茵陈蒿汤主要用于治疗肝炎、脂肪肝、肝硬化、黄疸等肝病，但其抗炎作用的分子机制尚不清楚。在这项研究中，我们研究了伴刀豆球蛋白A诱导小鼠T细胞依赖性肝炎的药理作用机制。伴刀豆球蛋白A给药导致严重肝损伤。这表现为血清转氨酶和乳酸脱氢酶水平升高，肝脏DNA片段化和caspase-3活性升高。茵陈蒿汤水提物预处理剂量依赖性地抑制转氨酶和乳酸脱氢酶活性的升高，降低肝DNA片段化和caspase-3水平。组织学改变有改善，包括炎性浸润、肝细胞坏死和变性以及枯否细胞增生。此外，茵陈蒿汤显着抑制肿瘤坏死因子-α（TNF-α）的生产在体外和体内。此外，茵陈蒿汤在体外和体内均能阻断调节TNF-α产生的核因子κ B（NF-κ B B）的活化。结论：茵陈蒿汤对T细胞介导的肝损伤具有一定的体内调节作用。该事件可能依赖于通过抑制NF-κ B活化降低TNF-α的产生。
In traditional oriental medicine, Yin-Chen-Hao decoction is used for the remedy of liver diseases such as hepatitis, fatty liver, hepatocirrhosis and jaundice, However, despite extensive pharmacological study, the molecular mechanism of the anti-inflammatory effect of Yin-Chen-Hao decoction is poorly understood. In this study, we have investigated the pharmacological action on the mechanism of concanavalin A-induced T cell-dependent hepatitis in mice. Concanavalin A administration resulted in a severe liver injury. This was shown through increased levels of serum transaminase and lactic dehydrogenase, and increased liver DNA fragmentation and caspase-3 activity. Pretreatment with the aqueous extract from Yin-Chen-Hao decoction dose-dependently inhibited the elevation in transaminase and lactic dehydrogenase activity, and reduced liver DNA fragmentation and caspase-3 levels. There was an improvement in histological changes including inflammatory infiltration, hepatocyte necrosis and degeneration, and Kupffer cell hyperplasia. In addition, Yin-Chen-Hao decoction significantly inhibited tumour necrosis factor-alpha (TNF-alpha) production in-vitro and in-vivo. Moreover, the activation of nuclear factor kappa B (NF-kappa B), which regulates TNF-alpha production, was blocked by Yin-Chen-Hao decoction in-vitro and in-vivo. In conclusion, Yin-Chen-Hao decoction was capable of regulating T-cell-mediated liver injury in-vivo. This event may have depended on the decrease of TNF-alpha production through the inhibition of NF-kappa B activation.