Antibacterial efficacy of silver-impregnated polyelectrolyte multilayers immobilized on a biological dressing in a murine wound infection model.
Antibacterial efficacy of silver-impregnated polyelectrolyte multilayers immobilized on a biological dressing in a murine wound infection model.
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DOI：
10.1097/sla.0b013e318256ff99
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发表时间：
2012-08
影响因子：
9
通讯作者：
McAnulty JF
中科院分区：
文献类型：
--
作者：
Guthrie KM;Agarwal A;Tackes DS;Johnson KW;Abbott NL;Murphy CJ;Czuprynski CJ;Kierski PR;Schurr MJ;McAnulty JF
探讨含银纳米粒子聚合物膜增强生物敷料的抗菌效果。生物敷料，如生物膜，通常用于治疗部分厚度的伤口和烧伤。与传统的伤口敷料相比，生物敷料有几个优点。然而，在使用生物膜治疗的伤口中，有多达19%的伤口会感染，一旦感染，必须移除生物膜，并采用传统的敷料方法。银是伤口护理产品中常用的抗菌剂，但目前的技术在这些敷料中使用细胞毒性浓度的银。我们已经开发了一种新颖而简单的技术，可以将生物活性分子固定在柔软材料的表面，这里通过纳米颗粒银增强生物膜来证明。用纳米厚的聚电解质多层膜（PEMs）浸渍银纳米颗粒修饰的表面，先前已被证明在无细胞毒性的银负载下对表皮葡萄球菌具有体外抗菌活性。我们证明了浸渍银的PEMs可以无损地固定在生物膜（生物膜- ag）表面，并测定了生物膜- ag对金黄色葡萄球菌的体外抗菌活性。在本研究中，我们采用局部接种金黄色葡萄球菌诱导小鼠全层创面感染模型。72h后，进行细菌定量。生物膜- ag处理的伤口菌落形成单位显著少于未修饰生物膜处理的伤口（差异大于4 log10）。我们的研究结果表明，在生物膜的创面上固定银浸渍的PEMs可以显著减少全层小鼠皮肤创面的细菌生物负荷。进一步的研究将调查这种结构是否可以被考虑用于人类。
To investigate the antibacterial effect of augmenting a biological dressing with polymer films containing silver nanoparticles. Biological dressings, such as Biobrane, are commonly used for treating partial-thickness wounds and burn injuries. Biological dressings have several advantages over traditional wound dressings. However, as many as 19% of wounds treated with Biobrane become infected, and, once infected, the Biobrane must be removed and a traditional dressing approach should be employed. Silver is a commonly used antimicrobial in wound care products, but current technology uses cytotoxic concentrations of silver in these dressings. We have developed a novel and facile technology that allows immobilization of bioactive molecules on the surfaces of soft materials, demonstrated here by augmentation of Biobrane with nanoparticulate silver. Surfaces modified with nanometer-thick polyelectrolyte multilayers (PEMs) impregnated with silver nanoparticles have been shown previously to result in in vitro antibacterial activity against Staphylococcus epidermidis at loadings of silver that are noncytotoxic. We demonstrated that silver-impregnated PEMs can be nondestructively immobilized onto the surface of Biobrane (Biobrane-Ag) and determined the in vitro antibacterial activity of Biobrane-Ag with Staphylococcus aureus. In this study, we used an in vivo wound infection model in mice induced by topical inoculation of S aureus onto full-thickness 6-mm diameter wounds. After 72 hours, bacterial quantification was performed. Wounds treated with Biobrane-Ag had significantly (P < 0.001) fewer colony-forming units than wounds treated with unmodified Biobrane (more than 4 log10 difference). The results of our study indicate that immobilizing silver-impregnated PEMs on the wound-contact surface of Biobrane significantly reduces bacterial bioburden in full-thickness murine skin wounds. Further research will investigate whether this construct can be considered for human use.