Multielemental distribution patterns in premature human milk whey and pre-term formula milk whey by size exclusion chromatography coupled to inductively coupled plasma mass spectrometry with octopole reaction cell
Multielemental distribution patterns in premature human milk whey and pre-term formula milk whey by size exclusion chromatography coupled to inductively coupled plasma mass spectrometry with octopole reaction cell
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DOI：
10.1039/b401527d
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发表时间：
2004-01-01
影响因子：
3.4
通讯作者：
Sanz-Medel, A
中科院分区：
文献类型：
--
作者：
St Remy, RRD;Sánchez, MLF;Sanz-Medel, A
在Superdex 200柱上进行的尺寸排阻色谱（SEC）已在线耦合到配备八极杆反应池（ORC）的四极杆ICP-MS仪器，用于早产儿母乳乳清和早产儿配方奶粉中几种必需元素（P、S、Cr、Mn、Fe、Co、Cu、Zn、Br、Se和I）和有毒元素（如Al）的多元素形态分析。将人乳样品在31000 g下离心以获得乳清。SEC-ICP-（ORC）MS联用技术进行了优化，然后应用于早产（妊娠28 - 32周后）人乳乳清中这些元素的多元素分馏在四个不同的泌乳阶段，涵盖出生后的第一个月（即：初乳，第6天，第14天和第28天的牛奶后交付）。同样，该技术也被应用于几种配方奶中的必需和有毒元素分馏，这些配方奶可用于喂养早产儿。上述元素在两个样本类型的研究（人类和配方奶）的分布模式的结果进行了讨论。对于许多必需元素（例如，维生素C、维生素E G. Cu、Fe和Zn）。从早产儿配方奶生产的角度强调了这种差异的营养意义和实际影响。
Size exclusion chromatography (SEC), carried out on a Superdex 200 column, has been coupled on line to a quadrupole ICP-MS instrument, equipped with an octopole reaction cell (ORC), for the multielemental speciation of several essential ( P, S, Cr, Mn, Fe, Co, Cu, Zn, Br, Se and I) and toxic elements ( such as Al) in premature human breast milk whey and premature infant formulae. Human milk samples were centrifuged at 31000g to obtain the milk whey. A SEC-ICP-(ORC) MS hyphenated technique was optimised and then applied to the multielemental fractionation of such elements in premature ( after 28 - 32 gestational weeks) human milk whey at four different lactating stages, covering the first month after birth ( namely: colostrum, 6th day, 14th day and 28th day milks after delivery). Similarly, the technique was also applied to essential and toxic elements fractionation in several formula milks, commercially available for feeding of premature newborns. Results of distribution patterns of the above mentioned elements in the two sample types under study ( human and formula milks) are discussed. Important differences in trace element speciation between premature human and formula milks have been demonstrated for many essential elements ( e. g. Cu, Fe and Zn). The nutritional significance and practical implications of such differences is highlighted from the point of view of formula milks production for premature babies.