Synchrotron µCT Investigation of the Collapsing Pore-Network of Gelatin Scaffolds under Compression
Synchrotron µCT Investigation of the Collapsing Pore-Network of Gelatin Scaffolds under Compression
复制标题
压缩下明胶支架塌陷孔网络的同步加速器 µCT 研究
DOI：
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
R. Zehbe
中科院分区：
文献类型：
--
作者：
A. Thiem;Victoria Seattle Lum;R. Grupp;H. Riesemeier;R. Bordia;H. Schubert;R. Zehbe
基于生物材料的组织工程需要优化几个参数。最重要的参数可归因于生物相容性、降解行为、机械稳定性和结构设计。在以前的研究中，我们已经建立了一种多孔明胶基支架材料，具有平行取向的孔道。虽然，断层扫描数据已得出干燥的支架样品，它仍然不清楚如何在潮湿的环境下的负载下的孔道网络相互作用。我们开发了一个实验装置，压缩生物材料在潮湿的环境中暴露于同步辐射产生的X射线使用测微螺钉与力传感器。在浸入水中的聚合物生物材料中实现良好的X射线吸收对比度是相当困难的，因为水的吸收阻止了详细的成像。另一方面，相衬成像由于成像数据中的相边界的衰减而允许改进的成像结果，几乎完全忽略了水相中的X射线吸收的影响。最好的结果，获得了30千电子伏的X射线能量与闪烁体样品的距离为1090毫米与建立的实验装置。由于在更高的光束能量下相边界的过度衰减，最终选择该能量。因此，我们可以评估塌陷孔隙网络加载后的可能性，以加强结构设计，为今后的研究。
Biomaterials based tissue engineering requires optimization of several parameters. The most important parameters can be attributed to biocompatibility, degradational behaviour, mechanical stability and structural design. In previous studies we have established a porous gelatine based scaffold material, with parallel oriented pore channels. Although, tomographic data has been derived on dried scaffold samples, it remains unclear how the pore channel network interacts under load in a wet environment. We developed an experimental setup to compress biomaterials in a wet environment during exposure to synchrotron generated X-rays using a micrometer screw with a force sensor. Achieving good X-ray absorption contrast in polymeric biomaterials immersed in water is rather difficult, as water absorption prevents detailed imaging. Phase contrast imaging on the other hand allows for improved imaging results due to the attenuation of phase boundaries in the imaged data, neglecting effects of X-ray absorption in the watery phase nearly completely. Best results were obtained for X-ray energies of 30 keV with a scintillator to sample distance of 1090 mm with the established experimental setup. Due to over attenuation of phase boundaries at higher beam energies, this energy was finally chosen. As a result, we could evaluate the collapsing pore network upon loading with the possibility to enhance the structural design for future studies.
影响因子：
7
作者：
Mow, VC;Wang, CC;Hung, CT
通讯作者：
Hung, CT
DOI：
10.1115/1.2894880
发表时间：
1991-08-01
影响因子：
1.7
作者：
LAI, WM;HOU, JS;MOW, VC
通讯作者：
MOW, VC
影响因子：
2.4
作者：
Guilak, F;Mow, VC
通讯作者：
Mow, VC