Alveolar liquid clearance in multiple nonperfused canine lung lobes.
Alveolar liquid clearance in multiple nonperfused canine lung lobes.
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多个非灌注犬肺叶的肺泡液体清除率。
DOI：
10.1152/jappl.1997.82.1.348
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
Maron,MB
中科院分区：
文献类型：
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作者：
Grimme,JD;Lane,SM;Maron,MB
格里姆，约翰D.，Susan M.莱恩和迈克尔B。Maron.多个非灌注犬肺叶中的肺泡液体清除率.J. Appl. Physiol.82（1）：348-353，1997.-我们通过同时测量从同一只犬中取出的三个肺叶的肺泡液体清除率（ALC），评价了犬离体非灌注肺叶从其空气空间吸收液体的能力。将自体血浆滴注到每个肺叶的空气空间中，并使用液体重吸收导致的血浆蛋白浓度增加来计算ALC。在基线条件下，4 h后的ALC为滴注液体体积的16.5 ± 0.6%（SE），在特布他林（10− 5 M）给药后为30.2 ± 1.3%。这些值与先前报告的完整犬的值相似。普萘洛尔（10− 4 M）和哇巴因（10− 3 M）分别将特布他林刺激的肺叶中的ALC降低至20.4 ± 0.8%和3.9 ± 1.4%。在基线条件下或特布他林给药后，三个肺叶之间的ALC无显著差异。这些数据表明，犬肺泡上皮细胞的钠和水的运输机制保持活力，在4小时的nonperfusion和有没有内在的差异，个别肺叶的运输性能。同时研究几个肺叶而不需要灌注的能力将允许设计实验，其中可以通过使用来自同一动物的肺叶来研究多种干预。
Grimme, John D., Susan M. Lane, and Michael B. Maron.Alveolar liquid clearance in multiple nonperfused canine lung lobes.J. Appl. Physiol.82(1): 348–353, 1997.—We evaluated the ability of canine isolated nonperfused lung lobes to absorb fluid from their air spaces by simultaneously measuring alveolar liquid clearance (ALC) in three lobes removed from the same dog. Autologous plasma was instilled in the air spaces of each lobe, and the increase in plasma protein concentration resulting from fluid reabsorption was used to calculate ALC. ALC after 4 h was 16.5 ± 0.6% (SE) of the instilled fluid volume under baseline conditions and was 30.2 ± 1.3% after terbutaline (10−5M) administration. These values were similar to those previously reported for intact dogs. Propranolol (10−4M) and ouabain (10−3M) reduced ALC in terbutaline-stimulated lobes to 20.4 ± 0.8 and 3.9 ± 1.4%, respectively. There was no significant difference in ALC among the three lobes under either baseline conditions or after terbutaline administration. These data indicate that the sodium and water transport mechanisms of the canine alveolar epithelium remain viable during 4 h of nonperfusion and that there are no intrinsic differences in the transport properties of individual lung lobes. The ability to study several lobes simultaneously without the need for perfusion will allow for the design of experiments in which multiple interventions can be studied by using lung lobes from the same animal.