Fine mapping of BrWax1, a gene controlling cuticular wax biosynthesis in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
Fine mapping of BrWax1, a gene controlling cuticular wax biosynthesis in Chinese cabbage (Brassica rapa L. ssp. pekinensis)
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BrWax1（一种控制大白菜（Brassica rapa L. ssp. pekinensis）角质层蜡生物合成的基因）的精细定位
DOI：
10.1007/s11032-013-9914-0
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发表时间：
2013-07
影响因子：
3.1
通讯作者：
刘志勇
中科院分区：
文献类型：
--
作者：
刘志勇
植物的表面覆盖着角质层蜡，其中含有极长链脂肪酸衍生物的混合物。这种蜡层提供了一个疏水屏障，减少非气孔水分损失，防止病原体的攻击。拟南芥表皮蜡的生物合成途径已被研究得很好，但对芜菁表皮蜡的合成却知之甚少。遗传分析表明，糯性性状受一对显性基因控制。在本研究中，初步定位结果表明，由308个隐性个体组成的F2群体BrWax 1（芸苔蜡）基因定位在连锁群A01。我们开发了一组与目标基因紧密连锁的新标记，并使用另一个1，020个隐性F2个体的群体将BrWax 1基因精细定位到约86.4 kb的基因组DNA片段。在该靶区域中鉴定了15个基因。基于基因注释，Bra013809基因是BrWax 1基因的候选者。实时荧光定量PCR分析和两个亲本的表达模式表明，Bra013809在蜡质表型中的表达水平远高于光泽表型。这一结果不仅为BrWax 1基因的功能研究提供了重要信息，也为研究芜菁表皮蜡质生物合成途径奠定了基础。
The surface of plants is covered with a cuticular wax, which contains a mixture of very-long-chain fatty acid derivatives. This wax layer provides a hydrophobic barrier which reduces non-stomatal water loss and prevents pathogen attack. The biosynthesis pathway of cuticular wax in Arabidopsis is well studied; however, little is known about the synthesis of cuticular wax in Brassica rapa. Genetic analyses indicated that the waxy characteristic is controlled by a single dominant gene. In the present study, preliminary mapping results from an F2 population consisting of 308 recessive individuals showed that the BrWax1 (Brassica Wax) gene is located on linkage group A01. We developed a set of new markers closely linked to the target gene, and used another population of 1,020 recessive F2 individuals to fine-map the BrWax1 gene to a genomic DNA fragment of approximately 86.4 kb. Fifteen genes were identified in this target region. Based on gene annotation, the Bra013809 gene was the candidate for the BrWax1 gene. Quantitative real-time PCR analysis and expression pattern of the two parents showed that the expression level of Bra013809 was much higher in the waxy phenotype than in the glossy phenotype. This result should provide not only important information for functional studies of the BrWax1 gene, but also the starting point for studying the pathway of cuticular wax biosynthesis in Brassica rapa.
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DOI：
10.2307/3871192
发表时间：
2000
期刊：
The Plant cell
影响因子：
--
作者：
C. Mariani;M. Wolters-Arts
通讯作者：
C. Mariani;M. Wolters-Arts
影响因子：
11.6
作者：
Fiebig, A;Mayfield, JA;Preuss, D
通讯作者：
Preuss, D
DOI：
10.2307/3241660
发表时间：
1971-01
期刊：
--
影响因子：
--
作者：
J. Martin;B. Juniper
通讯作者：
J. Martin;B. Juniper
影响因子：
4.3
作者：
Z. Ristić;M. Jenks
通讯作者：
Z. Ristić;M. Jenks
影响因子：
7.4
作者：
Xiaojie Xu;Charles R. Dietrich;R. Lessire;B. Nikolau;P. Schnable
通讯作者：
Xiaojie Xu;Charles R. Dietrich;R. Lessire;B. Nikolau;P. Schnable