Effects of rearing stress on Atlantic salmon (Salmo salar L.) antibody response to a non-pathogenic antigen
Effects of rearing stress on Atlantic salmon (Salmo salar L.) antibody response to a non-pathogenic antigen
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DOI：
10.1046/j.1365-2109.2000.00506.x
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发表时间：
2000-12-01
影响因子：
2
通讯作者：
Iversen, M
中科院分区：
文献类型：
--
作者：
Einarsdóttir, IE;Nilssen, KJ;Iversen, M
研究了急性应激对大西洋鲑鱼(Salmo salar L.)一抗和二抗反应的影响。FISH接受了绵羊红细胞(SRBC)的初次免疫，并在11周后接受了第二次免疫。在启动时，一组鱼被应激30min(中等应激)，另一组被应激两次，间隔6h，连续3d(重度应激)。在加强试验的时候，另外两组之前没有压力的、被启动的鱼也得到了同样的处理。未应激的鱼对SRBC有一次抗体应答和二次应答，与一次应答相比有明显的提高。在启动时，对SRBC的一次抗体应答受到严重(但不是中度)急性应激的影响，而二次抗体应答没有受到影响。在增强时，中度和重度应激对在非应激条件下准备的鱼都具有免疫抑制作用。结论是，在第二次免疫时，应激暴露导致的皮质醇释放对免疫抑制作用最强。
This study was carried out to test the effects of acute stress on the primary and the secondary antibody responses in Atlantic salmon (Salmo salar L.). Fish received a primary immunization with sheep red blood cells (SRBC) and received a second immunization 11 weeks later. At priming, a group of fish was stressed by water level reduction for 30 min (moderate stress), and another group was stressed severely by applying the stressor twice, with an interval of 6 h, 3 days in a row (severe stress). At the time of the booster, another two groups of previously unstressed, primed fish were treated likewise. Unstressed fish mounted a primary antibody response to SRBC and a secondary response, which was improved compared with the primary response., The primary antibody response against SRBC was impaired by severe, but not moderate, acute stress at priming, whereas the secondary antibody response was unaffected. Both moderate and severe stress at the time of the booster was immunosuppressive in fish that had been primed under non-stress conditions. It is concluded that cortisol release as a result of stress exposure is most immunosuppressive at the time of the second immunization.