Human leukocyte antigen matching estimations in a hypothetical bank of human embryonic stem cell lines in the Japanese population for use in cell transplantation therapy
Human leukocyte antigen matching estimations in a hypothetical bank of human embryonic stem cell lines in the Japanese population for use in cell transplantation therapy
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DOI：
10.1634/stemcells.2006-0566
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发表时间：
2007-01-01
期刊：
影响因子：
5.2
通讯作者：
Nakatsuji, Norio
中科院分区：
文献类型：
--
作者：
Nakajima, Fumiaki;Tokunaga, Katsushi;Nakatsuji, Norio
人胚胎干细胞(HES)细胞系在细胞移植治疗方面具有巨大潜力。然而，这种异基因移植的接受者可能需要免疫抑制剂的治疗。最近，泰勒等人提出了一个新的观点。[Lancet 2005；366：2019 2025]建议HES细胞系的银行，并估计在英国人群中有益的人类白细胞抗原(HL A)匹配所需的HES细胞系的数量。在这里，我们在日本人口中进行了这样的估计。我们计算了在人类白细胞抗原-A、-B和-DR三个基因座上至少能找到一个匹配的供者的患者的比例。从170个随机选择的捐赠胚胎中选择一组HES细胞系，80%的患者有望找到至少一个HES细胞系，在一个HL A座位上有一个单一的错配或更好的匹配。此外，如果从随机选择的55个捐献的卵母细胞中建立孤雌纯合子HES细胞系，80%的患者有望找到至少一个在三个人类白细胞抗原基因座上完全匹配的供者。日本人口的种族多样性相对较低，可能导致了有益配型的高成功率。此外，孤雌生殖的HES细胞系可以极大地减少更高程度的HLA配型所需的数量。
Human embryonic stem (hES) cell lines are of great potential in cell transplantation therapy. However, recipients of such allogeneic transplants probably need treatment with immunosuppressants. Recently, Taylor et al. [Lancet 2005; 366: 2019 2025] proposed banking of hES cell lines and estimated the required number of hES cell lines for beneficial human leukocyte antigen (HLA) matching in the U.K. population. Here, we carried out such an estimation in the Japanese population. We calculated the proportion of patients who can find at least one HLA-matched donor at three loci of HLA-A, -B, and -DR. With a bank of hES cell lines from 170 randomly selected donated embryos, 80% of patients were expected to find at least one hES cell line with a single mismatch at one HLA locus or a better match. Furthermore, 80% of patients are expected to find at least one donor with complete matching at the three HLA loci if parthenogenetic homozygous hES cell lines are established from 55 randomly selected donated oocytes. The relatively low ethnic diversity of the Japanese population may have resulted in a high success rate in beneficial matching. Moreover, parthenogenetic hES cell lines can greatly reduce the number required for a higher degree of HLA matching.