THE ROLE OF THE LOXP SPACER REGION IN P1 SITE-SPECIFIC RECOMBINATION
THE ROLE OF THE LOXP SPACER REGION IN P1 SITE-SPECIFIC RECOMBINATION
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DOI：
10.1093/nar/14.5.2287
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发表时间：
1986-03-11
影响因子：
14.9
通讯作者：
ABREMSKI, K
中科院分区：
文献类型：
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作者：
HOESS, RH;WIERZBICKI, A;ABREMSKI, K
细菌噬菌体P1的lox-Cre位点特异性重组系统由DNA上发生重组的位点（称为loxP）和介导反应的蛋白质Cre组成。loxP位点的长度为34个碱基对（bp），并且由两个13 bp的反向重复序列组成，所述反向重复序列由8 bp的间隔区隔开。先前已经表明，重组loxP位点的切割和链交换发生在该间隔区内。我们在这里报告的分析loxP间隔区内的各种碱基替换突变，并得出以下结论：（1）同源性是重组loxP位点之间有效重组的必要条件。(2)在间隔区内至少有一个位置，其中碱基变化大大减少了重组，即使在两个重组loxP位点之间存在同源性。(3)当含有对称间隔区的两个loxP位点在体外进行Cre介导的重组时，位点之间的DNA以相等的频率进行切除和倒位。
The lox-Cre site-specific recombination system of bacterio-phage P1 is comprised of a site on the DNA where recombination occurs called loxP, and a protein, Cre, which mediates the reaction. The loxP site is 34 base pairs (bp) in length and consists of two 13 bp inverted repeats separated by an 8 bp spacer region. Previously it has been shown that the cleavage and strand exchange of recombining loxP sites occurs within this spacer region. We report here an analysis of various base substitution mutations within the spacer region of loxP, and conclude the following: (l) Homology is a requirement for efficient recombination between recombining loxP sites. (2) There is at least one position within the spacer where a base change drastically reduces recombination even when there is homology between the two recombining loxP sites. (3) When two loxP sites containing symmetric spacer regions undergo Cre-mediated recombination in vitro, the DNA between the sites undergoes both excision and inversion with equal frequency.