Calcium-induced formation of chlorophyll b and light-harvesting chlorophyll ab-protein complex in cucumber cotyledons in the dark
Calcium-induced formation of chlorophyll b and light-harvesting chlorophyll ab-protein complex in cucumber cotyledons in the dark
复制标题
黑暗中黄瓜子叶中钙诱导叶绿素 b 和光捕获叶绿素 ab-蛋白复合物的形成
DOI：
10.1016/0005-2728(82)90138-4
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
H. Tsuji
中科院分区：
文献类型：
--
作者：
A. Tanaka;H. Tsuji
将黑暗生长的黄瓜幼苗暴露于间歇光照（2分钟光照和98分钟黑暗），然后将子叶与50 mM CaCl 2在黑暗中孵育。叶绿素（Chl）a选择性积累在间歇光照和Chl B积累在随后的黑暗培养与CaCl 2。采用SDS-聚丙烯酰胺凝胶电泳法研究了暗培养条件下CaCl 2诱导叶绿素B b积累过程中叶绿素蛋白复合物的变化。叶绿素-蛋白复合物I和游离叶绿素是间歇光照10次的子叶的主要含叶绿素带。当这些子叶与CaCl 2在黑暗中孵育时，形成捕光Chl a b-蛋白复合物。当间歇光照时间延长到55个时，在间歇光照结束时，叶绿素B和捕光叶绿素a b蛋白复合物被识别出来，并且这两种色素在随后的子叶与CaCl 2在黑暗中孵育过程中比水对照进一步增加。
Dark-grown cucumber seedlings were exposed to intermittent light (2 min light and 98 min dark) and then cotyledons were incubated with 50 mM CaCl 2 in the dark. Chlorophyll (Chl) a was selectively accumulated under intermittent light and Chl b was accumulated during the subsequent dark incubation with CaCl 2. The change in chlorophyll-protein complexes during Chl b accumulation induced by CaCl 2 in the dark was investigated by SDS-polyacrylamide gel electrophoresis. Chlorophyll-protein complex I and free chlorophyll were major chlorophyll-containing bands of the cotyledons intermittently illuminated 10 times. When these cotyledons were incubated with CaCl 2 in the dark, the light-harvesting Chl a b-protein complex was formed. When the number of intermittent illumination periods was extended to 55, small amounts of Chl b and light-harvesting Chl a b-protein complex were recognized at the end of intermittent light treatment, and these two pigments were further increased during the subsequent incubation of the cotyledons with CaCl 2 in the dark compared to water controls.