Dynamic regulation of circulating microRNA during acute exhaustive exercise and sustained aerobic exercise training
Dynamic regulation of circulating microRNA during acute exhaustive exercise and sustained aerobic exercise training
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DOI：
10.1113/jphysiol.2011.213363
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发表时间：
2011-08-15
影响因子：
5.5
通讯作者：
Chan, Stephen Y.
中科院分区：
文献类型：
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作者：
Baggish, Aaron L.;Hale, Andrew;Chan, Stephen Y.
microRNA（miRNAs）是细胞内重要的生物学功能的调节因子。最近，基于血浆的“循环”miRNA（c-miRNA）已被证明可以控制细胞过程，但c-miRNA对人类运动的反应仍然未知。我们试图确定c-miRNAs是否在急性力竭性自行车运动和持续划船运动训练中动态调节，使用纵向重复测量研究设计。具体来说，参与血管生成的c-miRNAs（miR-20 a、miR-210、miR-221、miR-222、miR-328）、炎症（miR-21，miR-146 a），骨骼肌和心肌收缩力（miR-21，miR-133 a）和缺氧/缺血适应在安静时和急性力竭性自行车运动后立即测量竞争性男性赛艇运动员的miR-21、miR-146 a和miR-210（n = 10，年龄= 19.1 +/- 0.6岁），在90天的划船训练之前和之后。观察到c-miRNA对运动的不同反应模式，并遵循四个主要特征：（1）持续训练前后急性运动上调c-miRNA表达（2）在持续训练之前但之后对急性运动有反应的c-miRNA（miR-146 a和miR-222），（miR-21和miR-221），（3）仅对持续训练有反应的c-miRNA（miR-20 a），和（4）无反应的c-miRNA（miR-133 a、miR-210、miR-328）。miR-146 a的峰值运动水平与（（V）超过点（O2 max））之间观察到线性相关性（r = 0.63，P = 0.003），静息miR-20 a的变化与（（V）超过点（O2 max））之间观察到线性相关性（训练前与训练后，r = 0.73; P = 0.02）。虽然需要进一步的研究，但这些结果表明c-miRNAs作为运动生物标志物的潜在价值及其作为运动诱导的心血管适应的生理介质的可能作用。
MicroRNAs (miRNAs) are intracellular mediators of essential biological functions. Recently, plasma-based 'circulating' miRNAs (c-miRNAs) have been shown to control cellular processes, but the c-miRNA response to human exercise remains unknown. We sought to determine whether c-miRNAs are dynamically regulated in response to acute exhaustive cycling exercise and sustained rowing exercise training using a longitudinal, repeated measures study design. Specifically, c-miRNAs involved in angiogenesis (miR-20a, miR-210, miR-221, miR-222, miR-328), inflammation (miR-21, miR-146a), skeletal and cardiac muscle contractility (miR-21, miR-133a), and hypoxia/ischaemia adaptation (miR-21, miR-146a, and miR-210) were measured at rest and immediately following acute exhaustive cycling exercise in competitive male rowers (n = 10, age = 19.1 +/- 0.6 years) before and after a 90 day period of rowing training. Distinct patterns of c-miRNA response to exercise were observed and adhered to four major profiles: (1) c-miRNA up-regulated by acute exercise before and after sustained training (miR-146a and miR-222), (2) c-miRNA responsive to acute exercise before but not after sustained training (miR-21 and miR-221), (3) c-miRNA responsive only to sustained training (miR-20a), and (4) non-responsive c-miRNA (miR-133a, miR-210, miR-328). Linear correlations were observed between peak exercise levels of miR-146a and ((V) over dot(O2max)) (r = 0.63, P = 0.003) and between changes in resting miR-20a and changes in ((V) over dot(O2max)) (pre-training vs. post-training, r = 0.73; P = 0.02). Although future work is required, these results suggest the potential value of c-miRNAs as exercise biomarkers and their possible roles as physiological mediators of exercise-induced cardiovascular adaptation.