System for preparing microhybridization arrays on glass slides.
System for preparing microhybridization arrays on glass slides.
复制标题
用于在载玻片上制备微杂交阵列的系统。
DOI：
10.1021/ac980456n
复制
发表时间：
1998
影响因子：
7.4
通讯作者：
Fortina,P
中科院分区：
文献类型：
--
作者：
Graves,DJ;Su,HJ;McKenzie,SE;Surrey,S;Fortina,P
我们描述了阵列系统的构建和操作，以产生用于分析用途的DNA序列模式，例如微芯片上的寡核苷酸微阵列。提供了详细的施工文件，以及增加的电子电路和软件的仪器，包括程序机加工自己的工作表面，以及那些操作它作为一个阵列。它的成本适中，并且使用单个液滴尖端，它可以在大约200分钟内在32个载玻片上每个载玻片上沉积存款96个点（容易升级到更高的速度）。按照目前的操作，它可以在1平方厘米内放置400个点，而且这个密度也可以很容易地增加。我们讨论的设计特点和性能，以证明实用性和灵活性。
We describe the construction and operation of an arrayer system to produce patterns of DNA sequences for analytical uses such as microarrays of oligonucleotide on microchips. Detailed documentation on construction is provided, as well as added electronic circuitry and the software for the instrument, including programs to machine its own working surface as well as those to operate it as an arrayer. Its cost is modest, and with a single droplet tip it can deposit 96 spots per slide on 32 slides in about 200 min (readily upgraded to higher speeds). As currently operated, it can place 400 spots in 1 cm2, and this density, too, can be increased easily. We discuss design features and performance to demonstrate utility and flexibility.