Differential wound induction of the myrosinase system in oilseed rape (Brassica napus):: contrasting insect damage with mechanical damage
Differential wound induction of the myrosinase system in oilseed rape (Brassica napus):: contrasting insect damage with mechanical damage
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DOI：
10.1016/j.plantsci.2004.10.003
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发表时间：
2005-03-01
期刊：
影响因子：
5.2
通讯作者：
Meijer, J
中科院分区：
文献类型：
--
作者：
Pontoppidan, B;Hopkins, R;Meijer, J
小菜蛾是一种严重的害虫，专用于十字花科植物上的草食，尽管这类植物含有硫代葡萄糖苷-黑芥子酶系统，是抵抗害虫的重要资源。对油菜(Brassica Napus)进行了用钳子或剪刀机械伤害和小菜蛾幼虫食草性伤害的效果比较。研究了不同时间点叶片中黑芥子酶结合蛋白(MBP)、黑芥子酶相关蛋白(MyAP)和黑芥子酶的转录水平，并测定了黑芥子酶的活性。伤后24~48h，MBP和MyAP的转录水平均出现一过性的局部升高，并在24~48h内恢复到与对照组相似的水平，但剪刀和剪刀致伤后，MBP和MyAP的转录水平发生了系统性的变化，而钳子致伤后MBP和MyAP的转录水平下降。相反，取食小菜蛾后，黑芥子酶转录水平的诱导较少，仅在受伤的叶片中发生。除草后，芥子酶活性下降，尤以伤叶的可溶性部分最为明显。根据蛋白质印迹分析，MBP和芥子酶转录水平的变化反映在蛋白质水平上。为了方便起见，经常使用钳子进行机械伤害，以代替害虫的攻击，但并不能很好地模拟小菜蛾对植物的食草性影响。(C)2004爱思唯尔爱尔兰有限公司。保留所有权利。
Diamond-back moth (Plutella xylostella) is a serious insect pest specialised for herbivory on Crucifers although such plants contains the glucosinolate-myrosinase system implied as an important resource to withstand insect pests. We have compared the effects of mechanical wounding using forceps or scissors with herbivory by diamond-back moth larvae on oilseed rape (Brassica napus). The transcript levels of myrosinase binding protein (MBP), myrosinase associated protein (MyAP) and myrosinase were studied of leaf samples at different time points together with myrosinase enzyme activity measurements. In all cases, wounding gave a transient local increase in transcript levels of MBP and MyAP that were similar to control levels again within 24-48 h. Systemic induction of MBP and MyAP transcripts was observed after diamond-back moth herbivory and mechanical Wounding by scissors while wounding by forceps caused down-regulation. In contrast, the myrosinase transcript levels were induced less and in wounded leaves after diamond-back moth feeding only. The myrosinase activity decreased especially in the soluble fraction prepared from wounded leaves after herbivory. The changes in MBP and myrosinase transcript levels were reflected in protein levels according to Western blot analysis. Mechanical wounding with forceps is often used for convenience as a substitute for insect attack but does not mimic well the effects of diamond-back moth herbivory to the plants. (C) 2004 Elsevier Ireland Ltd. All rights reserved.