Increased 8-isoprostane, a marker of oxidative stress, in exhaled condensate of asthma patients
Increased 8-isoprostane, a marker of oxidative stress, in exhaled condensate of asthma patients
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DOI：
10.1164/ajrccm.160.1.9809140
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发表时间：
1999-07-01
影响因子：
24.7
通讯作者：
Barnes, PJ
中科院分区：
文献类型：
--
作者：
Montuschi, P;Corradi, M;Barnes, PJ
氧化应激在哮喘的发病机制中具有重要作用。8-异前列烷是属于F-2异前列烷类的前列腺素（PG）-F-2样化合物，其通过花生四烯酸的自由基催化过氧化在体内产生。8-异前列烷是氧化应激的生物标志物，其浓度在间质性肺病患者的支气管肺泡灌洗液中增加。我们测量了健康受试者和轻度（类固醇初治，n = 12）、中度（吸入类固醇治疗，n = 17）和重度哮喘（口服类固醇治疗，n = 15）患者呼出气冷凝液中的8-异前列烷浓度。我们还测量了呼出的一氧化碳（CO）和一氧化氮（NO），这也可以反映气道中的氧化应激。8-正常人呼气冷凝液中可检出异前列烷（15.8 +/- 1.6 pg/ml），在轻度呼吸困难患者的呼吸冷凝液中增加（33.7 ± 2.8，p < 0.001），中度（38.3 ± 3.7 pg/ml，p < 0.001）和重度哮喘（48.9 ± 5.0 pg/ml，p < 0.001）。轻度哮喘患者呼出气中8-异前列腺素与NO呈正相关（r = 0.68，p < 0.05），与CO无相关性（r = 0.05，p> 0.05），而中重度哮喘患者呼出气中8-异前列腺素与CO无相关性。在任何患者组中，8-异前列腺素和肺功能检查之间均无相关性。我们的研究表明，哮喘患者的氧化应激增加，反映在呼气冷凝液中的8-异前列烷浓度。
Oxidative stress has an important role in the pathogenesis of asthma. 8-Isoprostane is a prostaglandin (PG)-F-2-like compound belonging to the F-2 isoprostane class that is produced in vivo by the free radical-catalyzed peroxidation of arachidonic acid. 8-Isoprostane is a biomarker of oxidative stress, and its concentration is increased in the bronchoalveolar lavage fluid of patients with interstitial lung diseases. We measured 8-isoprostane concentrations in exhaled breath condensate in healthy subjects and in patients with mild (steroid naive, n = 12), moderate (inhaled steroid treatment, n = 17), and severe asthma (oral steroid treatment, n = 15). We also measured exhaled carbon monoxide (CO) and nitric oxide (NO), which may also reflect oxidative stress in the airways. 8-Isoprostane was detectable in breath condensate of normal subjects (15.8 +/- 1.6 pg/ml), and was increased in the breath condensate of patients with mild (33.7 +/- 2.8, p < 0.001), moderate (38.3 +/- 3.7 pg/ml, p < 0.001), and severe asthma (48.9 +/- 5.0 pg/ml, p < 0.001). There was a positive correlation (r = 0.68, p < 0.05) of 8-isoprostane with NO, but not with CO, in the exhaled air of patients with mild asthma, but not in that of patients with moderate or severe asthma. There was no correlation between 8-isoprostane and lung function tests in any group of patients. Our study shows that oxidative stress is increased in asthmatic subjects as reflected by 8-isoprostane concentrations in breath condensate.