CD8+ T cells rapidly acquire NK1.1 and NK cell-associated molecules upon stimulation in vitro and in vivo
CD8+ T cells rapidly acquire NK1.1 and NK cell-associated molecules upon stimulation in vitro and in vivo
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DOI：
10.4049/jimmunol.165.7.3673
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发表时间：
2000-10-01
影响因子：
4.4
通讯作者：
Chambers, BJ
中科院分区：
文献类型：
--
作者：
Assarsson, E;Kambayashi, T;Chambers, BJ
NKT细胞表达NK细胞相关标志物和TCR。传统上，这些NK1.1（+）TCR α β（+）细胞被描述为CD 4（+）CD 8（-）或CD 4（-）CD 8（-）。大多数NKT细胞通过基本不变的V α 14-J α 281 TCR链结合V β 2、-7或-8 TCR链与非经典MHC I类分子CD 1相互作用。在本研究中，我们描述了在缺乏经典NKT细胞的野生型C57 BL/6、CD 1d 1（-/-）和J α 281（-/-）小鼠的淋巴因子激活的杀伤细胞培养物中存在大量NK1.1（+）TCR α β（+）细胞。与经典的NKT细胞不同，这些NK1.1（+）TCR α β（+）细胞中的50-60%表达CD 8并具有不同的TCR V β库。来自B6小鼠脾脏的纯化NK1.1（-）CD 8 α（+）T细胞在体外用IL-2、IL-4或IL-15刺激后，迅速获得NK1.1的表面表达。许多NK 1.1（+）CD 8（+）T细胞也获得了Ly-49受体和其他NK细胞相关分子的表达。在CD 8（+）T细胞上获得NK1.1表达是CD 8（+）T细胞的IL-2 R β（+）亚群的特殊性质。在CD 8（+）T细胞上有效的NK1.1表达需要Lck而不是Fyn。在CD 8（+）T细胞上NK1.1的诱导不仅仅是体外现象，因为我们观察到在流感病毒感染的小鼠的肺中NK1.1（+）CD 8（+）T细胞增加了5倍。这些数据表明，在体外和体内适当刺激下，CD 8（+）T细胞可以获得NK 1.1和其他NK细胞相关分子。
NKT cells express both NK cell-associated markers and TCR, Classically, these NK1.1(+)TCR alpha beta(+) cells have been described as being either CD4(+)CD8(-) or CD4(-)CD8(-). Most NKT cells interact with the nonclassical MHC class I molecule CD1 through a largely invariant V alpha 14-J alpha 281 TCR chain in conjunction with either a V beta 2, -7, or -8 TCR chain. In the present study, we describe the presence of significant numbers of NK1.1(+)TCR alpha beta(+) cells within lymphokine-activated killer cell cultures from wild-type C57BL/6, CD1d1(-/-), and J alpha 281(-/-) mice that lack classical NKT cells. Unlike classical NKT cells, 50-60% of these NK1.1(+)TCR alpha beta(+) cells express CD8 and have a diverse TCR V beta repertoire. Purified NK1.1(-)CD8 alpha(+) T cells from the spleens of B6 mice, upon stimulation with IL-2, IL-4, or IL-15 in vitro, rapidly acquire surface expression of NK1.1. Many NK1.1(+)CD8(+) T cells had also acquired expression of Ly-49 receptors and other NK cell-associated molecules. The acquisition of NK1.1 expression on CD8(+) T cells was a particular property of the IL-2R beta(+) subpopulation of the CD8(+) T cells. Efficient NK1.1 expression on CD8(+) T cells required Lck but not Fyn, The induction of NK1.1 on CD8(+) T cells was not just an in vitro phenomenon as we observed a 5-fold increase of NK1.1(+)CD8(+) T cells in the lungs of influenza virus-infected mice. These data suggest that CD8(+) T cells can acquire NK1.1 and other NK cell-associated molecules upon appropriate stimulation in vitro and in vivo.