Molecular cloning of integrated simian sarcoma virus: genome organization of infectious DNA clones.
Molecular cloning of integrated simian sarcoma virus: genome organization of infectious DNA clones.
复制标题
整合猿肉瘤病毒的分子克隆：感染性 DNA 克隆的基因组组织。
DOI：
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发表时间：
1981
影响因子：
11.1
通讯作者：
S. Aaronson
中科院分区：
文献类型：
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作者：
K. Robbins;S. Devare;S. Aaronson
猿肉瘤病毒（SSV）的整合形式被分子克隆到噬菌体 lambda 的 Charon 16A 株中。在转染分析中，重组病毒 DNA 表现出高效转化组织培养细胞的能力。此类转化体具有典型的SSV形态，在没有病毒释放的情况下表达猿肉瘤相关病毒（SSAV）gag基因产物，并在用C型辅助病毒重复感染后释放SSV。根据限制性内切核酸酶分析推导出 5.8 kbp 重组病毒 DNA 克隆的物理图谱，揭示了 5.1 kbp SSV 基因组，其中包含 0.55 kbp 长的末端重复序列，两侧是 0.45 和 0.25 kbp 的连续宿主细胞序列。通过 R 环分析，病毒 DNA 分子包含两个与 SSAV 同源的区域，由 1.0 kbp 的非同源区域隔开。该 SSV 特异性序列被证明在包括人类在内的不同哺乳动物物种的正常细胞 DNA 中具有独特的代表性。我们的结果表明，这种灵长类转化逆转录病毒在自然界中是由 C 型辅助病毒和宿主细胞基因重组产生的。
The integrated form of simian sarcoma virus (SSV) was molecularly cloned in the Charon 16A strain of bacteriophage lambda. In transfection analysis, the recombinant viral DNAs demonstrated the ability to transform cells in tissue culture at high efficiency. Such transformants possessed typical SSV morphology, expressed simian sarcoma associated virus (SSAV) gag gene products in the absence of virus release, and released SSV after superinfection with a type C helper virus. A physical map of the 5.8-kilobase-pair (kbp) recombinant viral DNA clone, deduced from restriction endonuclease analysis, revealed a 5.1-kbp SSV genome containing 0.55-kbp-long terminal repeats flanked by 0.45 and 0.25 kbp of contiguous host cell sequences. By R-loop analysis, the viral DNA molecule contained two regions of homology to SSAV, separated by a 1.0-kbp nonhomologous region. This SSV-specific sequence was shown to be uniquely represented within the normal cellular DNA of diverse mammalian species, including human. Our results demonstrate that this primate transforming retrovirus arose in nature by recombination of a type C helper virus and a host cellular gene.