Rigidity of triskelion arms and clathrin nets
Rigidity of triskelion arms and clathrin nets
复制标题
DOI：
10.1016/s0006-3495(00)76676-8
复制
发表时间：
2000-03-01
影响因子：
3.4
通讯作者：
Nossal, R
中科院分区：
文献类型：
--
作者：
Jin, AJ;Nossal, R
对一组电子显微图像（Kocsis，E，，B. L. Trus，C，J. Steer，M. E，Bisher，和A，C。史蒂文. 1991. 107：6 - 14），从其中获得定量测量，以支持三臂的三个臂具有统计上相同的属性，并表现出独立的结构波动的概念。此外，局部轮廓波动的研究表明，三臂臂的弹性特性在整个臂长上近似恒定，该研究与小变形统计力学理论一起沿着用于导出臂的有效平均抗弯刚度。该结果用于估计使网格蛋白贴片变形所需的弯曲能量，并与非网格蛋白涂覆的膜的等效面积的变形能量进行比较。我们估计网格蛋白晶格的刚性至少与典型膜的刚性相当。因此，网格蛋白笼的自然曲率可以稳定，并可能推动细胞内包被囊泡的形成。
Statistical analysis is applied to a set of electron micrographic images (Kocsis, E,, B. L. Trus, C, J. Steer, M. E, Bisher, and A, C. Steven. 1991. J. Struct. Biol. 107:6-14), from which quantitative measures are obtained to support the notion that the three arms of a triskelion have statistically identical properties and exhibit independent structural fluctuations. additionally, a study of local contour fluctuations, which indicates that the elastic properties of a triskelion arm are approximately constant over the entire arm length, is used along with a small deformation statistical mechanics theory to derive an:effective, average flexural rigidity for the arms. This result is used to estimate the bending energy necessary to deform a clathrin patch, and comparison is made with the deformation energy of an equivalent area of non-clathrin-coated membrane. We estimate that the rigidity of the clathrin lattice is at least comparable to that of a typical membrane. Hence, the natural curvature of a clathrin cage can stabilize, and perhaps propel, the formation of intracellular coated vesicles.