Fission of biological membranes: Interplay between dynamin and lipids
Fission of biological membranes: Interplay between dynamin and lipids
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DOI：
10.1034/j.1600-0854.2001.020107.x
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发表时间：
2001-01-01
期刊：
影响因子：
4.5
通讯作者：
Kozlov, MM
中科院分区：
文献类型：
--
作者：
Kozlov, MM
膜出芽和分裂是细胞内转运囊泡形成过程的关键阶段。我们提出了一个理论上的考虑，最重要的类型之一的裂变机制，这是介导的GTdR发动蛋白和控制膜的脂质成分。我们提出了一种机制，崩溃的膜颈部之间的相互作用驱动的发动蛋白衣领和颈部膜的弯曲弹性能量。衣领扮演着一个刚性的外部骨架的角色，它对颈部施加了机械约束。我们表明，在某些条件下，颈部的膜失去了稳定性和崩溃。塌陷可能由以下原因引起：ii）颈膜的自发曲率向负值移动，iii）发动蛋白领的拉伸，（iii）发动蛋白领的收紧。这三个因素可以单独或协同作用。该模型解释了发动蛋白介导的膜分裂的主要实验知识。它包括基于不同实验结果的所有先前发动蛋白作用模型的元素[Sever等人，Traffic 2000; 1：385-392]。它至少部分地调和了现有的关于生物膜裂变机制的不同观点之间的明显矛盾。
Membrane budding and fission are the key stages of ubiquitous processes of formation of intracellular transport vesicles. We present a theoretical consideration of one of the most important types of fission machinery, which is mediated by GTPase dynamin and controlled by lipid composition of the membrane. We suggest a mechanism for collapse of a membrane neck driven by interplay between the dynamin collar and the bending elastic energy of the neck membrane. The collar plays a role of a rigid external skeleton, which imposes mechanical constraints on the neck. We show that in certain conditions the membrane of the neck loses its stability and collapses. Collapse can result from: ii) shifting of the spontaneous curvature of the neck membrane towards negative values, iii) stretching of the dynamin collar, (iii) tightening of the dynamin collar. The three factors can act separately or concertedly. The suggested model accounts for the major experimental knowledge on membrane fission mediated by dynamin. It includes the elements of all previous models of dynamin action based on different sets of experimental results [Sever et al., Traffic 2000; 1: 385-392]. It reconciles, at least partially, the apparent contradictions between the existing alternative views on biomembrane fission machinery.