Kinetic analysis of respiratory tract proteins recovered during a sequential lavage protocol.
Kinetic analysis of respiratory tract proteins recovered during a sequential lavage protocol.
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对连续灌洗方案中回收的呼吸道蛋白质进行动力学分析。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
H. Reynolds
中科院分区：
文献类型：
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作者：
W. Merrill;E. O'Hearn;J. Rankin;G. Naegel;R. Matthay;H. Reynolds
尽管支气管肺泡灌洗（BAL）已被用作研究工具十多年，但灌洗技术在实验室之间存在显着差异。例如，已经使用了50至300 ml的灌洗滴注体积，但是总灌洗体积的变量对随后的蛋白质回收的影响是不确定的。我们对14名正常志愿者的右中叶进行了连续BAL（50 ml/等份;总体积，300 ml），并分别处理和分析了回收的等份样品中几种蛋白质物质的绝对和相对浓度。这些蛋白质包括游离分泌组分和分泌型伊加，其源自气道分泌物，以及IgG，其被认为是从更远的肺泡部位漏出。对这些数据的分析显示，在连续等分试样中测量的所有蛋白质的绝对浓度显著降低。然而，连续等分试样中的蛋白质比率分析显示，从第一份到第五份回收等分试样无显著变化。最后，我们分析了第一个回收的等分试样的大小对蛋白质的绝对和相对浓度的影响。此处似乎存在一种趋势，表明气道蛋白以较小的等分试样优先回收。这对于游离分泌组分与白蛋白的比率是显著的（p小于0.05）。我们的结论是，肺蛋白质的有效和均匀采样与100毫升灌洗液滴注。更大的体积会增加更多的蛋白质，但不会改变蛋白质比例。较小体积的灌洗可优先对气道蛋白进行采样。
Although bronchoalveolar lavage (BAL) has been used as a research tool for over a decade, the technique of lavage has varied markedly between laboratories. For example, lavage instillate volumes from 50 to 300 ml have been used, and yet the influence of the variable of total lavage volume on subsequent protein recovery is uncertain. We performed sequential BAL (50 ml/aliquot; total volume, 300 ml) of the right middle lobe of 14 normal volunteers and separately processed and analyzed recovered aliquots for the absolute and relative concentrations of several protein substances. These proteins include free secretory component and secretory IgA, which emanate from airway secretions, and IgG, which is thought to transude from more distal alveolar sites. Analysis of these data showed a marked decrease in the absolute concentration of all proteins measured in serial aliquots. Analysis of protein ratios in sequential aliquots, however, revealed no significant change from the first to the fifth recovered aliquot. Finally, we analyzed the influence of the size of the first recovered aliquot on absolute and relative concentrations of proteins. Here there seemed to be a trend indicating preferential recovery of airway proteins in smaller aliquots. This was significant for the ratio of free secretory component to albumin (p less than 0.05). We conclude that lung proteins are efficiently and homogeneously sampled with 100 ml of lavage instillate. Larger volumes will add more protein but not alter protein ratios. Lavage with smaller volumes may preferentially sample airway proteins.