Urethral musculature and innervation in the female rat.
Urethral musculature and innervation in the female rat.
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DOI：
10.1002/nau.22722
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发表时间：
2016-03
影响因子：
2
通讯作者：
Lue TF
中科院分区：
文献类型：
--
作者：
Zhang X;Alwaal A;Lin G;Li H;Zaid UB;Wang G;Wang L;Banie L;Ning H;Lin CS;Guo Y;Zhou L;Lue TF
尿道括约肌和尿道肌肉神经支配对于维持节制至关重要，尤其是女性。然而，尿道肌肉的类型和分布以及尿道神经还远没有得到很好的记录。我们的目的是清楚地识别尿道横纹肌、平滑肌和尿道神经的分布。在对 3 个月大雌性 Sprague-Dawley 大鼠进行的队列分析中，使用形态学技术对雌性大鼠尿道的横向和纵向切片进行了广泛研究。将尿道切成切片，以提供尿道的整体和详细视图。使用 H&E、Masson Trichrome、鬼笔环肽和免疫荧光染色剂。主要研究细胞结构、氮能和胆碱能神经支配。追踪尿道各节段的不同层，绘制代表尿道壁各肌层厚度的曲线图。结果表明，横纹肌的主峰位于尿道中段。发现靠近粘膜的内层含有纵向平滑肌。在膀胱口附近，圆形平滑肌占主导地位，在尿道其余部分的远端逐渐变薄。在尿道中部，绝大多数尿道肌是环状定向的横纹肌细胞。典型的神经末梢出现在高倍图像中，以显示神经支配的不同特征。本研究阐明了正常雌性大鼠尿道的详细形态结构和神经支配，可为进一步研究压力性尿失禁（SUI）奠定基础。
The urethral sphincter and urethral muscle innervation are critically involved in maintaining continence, especially in the female. However, the urethral muscle type and distribution, as well as the urethral nerves are far from being well documented. Our aim was to clearly identify the distribution of urethral striated muscle, smooth muscle, and urethral nerves. In a cohort analysis of 3-month-old female Sprague-Dawley rats, cross and longitudinal sections of female rat urethra were extensively investigated using morphological techniques. Urethras were harvested to the sections, in order to provide both global and detailed visions of the urethra. H&E, Masson’s Trichrome, phalloidin and immunoflourence stains were used. The cytoarchitecture, nitrergic, and cholinergic innervations were mainly investigated. Different layers of the segments of urethra were traced to draw curve graphs that represent the thickness of each muscle layer of urethral wall. The results showed that the primary peak of striated muscle is in the middle urethra. The inner layer close to mucosa was found to contain longitudinal smooth muscle. Near the bladder orifice, the circular smooth muscle dominates, which becomes thinner distally throughout the rest of urethra. In the middle urethra the vast majority of the urethral muscle are circularly oriented striated muscle cells. Typical nerve endings were present in high power images to show the different characteristic features of nerve innervation. This study has illustrated the detailed morphological structure and innervations of the normal female rat urethra can serve as a basis for further study for stress urinary incontinence (SUI).