Origins of vaginal acidity: high D/L lactate ratio is consistent with bacteria being the primary source
Origins of vaginal acidity: high D/L lactate ratio is consistent with bacteria being the primary source
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DOI：
10.1093/humrep/16.9.1809
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发表时间：
2001-09-01
影响因子：
6.1
通讯作者：
Moench, TR
中科院分区：
文献类型：
--
作者：
Boskey, ER;Cone, RA;Moench, TR
背景：使阴道酸化的乳酸的来源还没有很好的确定。人们普遍认为，在高雌激素时期（在新生儿期和妇女生育期），大量糖原沉积在阴道上皮中，糖原被厌氧代谢为乳酸。目前尚不确定的是乳酸主要是由阴道细菌还是阴道上皮细胞产生的。人类细胞只能产生L-乳酸，而细菌可以产生D-和L-，因此D-乳酸与L-乳酸的比例可以指示细菌来源的乳酸的相对贡献。方法：在这项研究中，我们使用手性HPLC来检查阴道分泌物中的D-和L-乳酸的百分比，在这些阴道分泌物的细菌的原代培养物中，以及在阴道来源的细菌分离株的培养物中。结果：我们发现，在大多数阴道分泌物样本中，>50%的乳酸是D-亚型（平均55%，范围6- 75%，n = 14）。结论：因此，我们的结果支持了这样的假设：阴道细菌而不是上皮细胞是阴道中乳酸的主要来源。
BACKGROUND: The origin of the lactic acid that acidifies the vagina is not well established. It is widely accepted that during times of high oestrogen (during the neonatal period and again during a woman's reproductive years) large amounts of glycogen are deposited in the vaginal epithelium and that the glycogen is anaerobically metabolized to lactic acid. What is not established is whether lactic acid is primarily produced by vaginal bacteria or by vaginal epithelial cells. Human cells can make only L-lactate, while bacteria can produce both D- and L-, thus the D- to L-lactate ratio can indicate the relative contribution of bacterially derived lactic acid. METHODS: In this study, we used chiral HPLC to examine the percentages of D- and L-lactate in vaginal secretions, in primary cultures of bacteria from these vaginal secretions, and in cultures of lactobacillus isolates of vaginal origin. RESULTS: We found that in most vaginal secretion samples, >50% of the lactic acid was the D-isoform (mean 55%, range 6-75%, n = 14). CONCLUSIONS: Our results thus support the hypothesis that vaginal bacteria, not epithelial cells, are the primary source of lactic acid in the vagina.