Continuous antigen delivery from controlled release implants induces significant and anamnestic immune responses
Continuous antigen delivery from controlled release implants induces significant and anamnestic immune responses
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DOI：
10.1016/s0264-410x(01)00444-3
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发表时间：
2002-01-15
期刊：
影响因子：
5.5
通讯作者：
Lofthouse, S
中科院分区：
文献类型：
--
作者：
Kemp, JM;Kajihara, M;Lofthouse, S
对两种连续注射的硅胶植入物进行了测试，以确定它们是否能够一次注射疫苗。A型植入物在体外1个月内输送抗原，而B型植入物在几个月内输送抗原。在绵羊上进行的疫苗接种研究旨在比较A型和B型植入物、阿尔赛特(TM)微型渗透泵和传统抗原输送引起的反应。一种模型抗原，亲和素，与IL-Ibeta或明矾一起用作佐剂。用不同的配方免疫绵羊，并监测抗原特异性抗体的效价和同型。与可溶性疫苗或带有佐剂的A型植入物相比，B型植入物诱导的抗体(Ab)滴度更大，持续时间更长，但前提是配方中包含IL-1β。两种植入物均可产生IgG1和IgG2同型抗体。B型+IL-1β植入物可诱导对可溶性抗原攻击的记忆反应，该植入物主要为IgG1同型。(C)2002爱思唯尔科学有限公司。保留所有权利。
Two continuous delivery injectable silicone implants were tested to determine if they were capable of delivering vaccines in a single shot. The Type A implant delivers antigen in vitro over a 1-month-period and the Type B over several months. Vaccination studies in sheep were designed to compare the responses induced by the Type A and B implants, Alzet(TM) mini-osmotic pumps and conventional antigen delivery. A model antigen, avidin, was used along with IL-Ibeta or alum as adjuvants. Sheep were immunised with various formulations and the titre and isotype of the antigen specific antibodies monitored. The Type B implant induced antibody (Ab) titres of greater magnitude and duration than soluble vaccines or the Type A implant with adjuvant, but only if IL-1beta was included in the formulation. Both implants induced antibodies of IgG1 and IgG2 isotype. A memory response to soluble antigen challenge was induced by the Type B+IL-1beta implant, which was predominantly of an IgG1 isotype. (C) 2002 Elsevier Science Ltd. All rights reserved.