Development of novel thioguanosine analogs with the ability to specifically modify cytidine.
Development of novel thioguanosine analogs with the ability to specifically modify cytidine.
复制标题
开发能够特异性修饰胞苷的新型硫鸟苷类似物。
DOI：
10.1093/nass/nrm003
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
S. Sasaki
中科院分区：
文献类型：
--
作者：
Kazumitsu Onizuka;Y. Taniguchi;S. Sasaki
核酸的位点特异性修饰在基因表达机制中具有重要意义。通过掺入S-乙烯基硫鸟苷类似物的寡核苷酸对核酸进行特异性修饰，作为有用的工具具有巨大的潜力。乙烯基衍生物可特异性转移至互补 ODN 靶位点 dC 的氨基上。这是一种高效、选择性修饰 DNA 中靶胞苷的创新方法。
Site-specific modification of nucleic acid is of great significance in the machinery of gene expression. Specific modification of nucleic acids by an oligonucleotide incorporating a S-vinyl thioguanosine analog, has great potential as a useful tool. The specific transfer of the vinyl derivative to the amino group of dC at the target site of the complementary ODN has been demonstrated. This is an innovative method for the efficient and selective modification of the target cytidine in DNA.