GENE ENCODING CAPSID PROTEIN VP1 OF FOOT-AND-MOUTH-DISEASE VIRUS - A QUASISPECIES MODEL OF MOLECULAR EVOLUTION
GENE ENCODING CAPSID PROTEIN VP1 OF FOOT-AND-MOUTH-DISEASE VIRUS - A QUASISPECIES MODEL OF MOLECULAR EVOLUTION
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DOI：
10.1073/pnas.85.18.6811
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发表时间：
1988-09-01
影响因子：
11.1
通讯作者：
MOYA, A
中科院分区：
文献类型：
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作者：
DOPAZO, J;SOBRINO, F;MOYA, A
建立了15株甲型、丙型和O型口蹄疫病毒VP1基因的系统发育树。最简约树显示，FMDV亚型和亚型内的分离株构成了相关的、不相同的基因组集，这与该病毒的准种进化模式一致。在血清型A的代表中，所有可能的VP1编码片段对的每个位点的平均核苷酸替换数高于血清型C或o。在比较氨基酸序列时，分散指数（方差/平均值）的值为>.1，在考虑所有序列时得分最高。这表明突变在有限数量的残基上积累，表明序列分布在高稳定性点附近波动。FMDV的进化遵循与恒星系统发育非常遥远的路径，并且不可能通过应用于分析的测试得出进化速率恒定的结论。与其他RNA病毒一样，FMDV的遗传复杂性有限，其种群规模非常大。它们的进化涉及复杂的、不确定的基因组混合物，而不是单一的、确定的物种。
A phylogenetic tree relating the VP1 gene of 15 isolates of foot-and-mouth disease virus (FMDV) of serotypes A, C, and O has been constructed. The most parsimonious tree shows that FMDV subtypes and isolates within subtypes constitute sets of related, nonidentical genomes, in agreement with a quasispecies mode of evolution of this virus. The average number of nucleotide replacements per site for all possible pairs of VP1 coding segments is higher among representatives of serotype A than serotype C or O. In comparing amino acid sequences, the values of dispersion index (variance/mean value) are >1, with the highest values scored when all sequences are considered. This indicates an accumulation of mutations at a limited number of residues, suggesting that distributions of sequences fluctuate around points of high stability. Evolution of FMDV follows a path very distant from that of a star phylogeny, and it has not been possible to derive conclusions on constancy of evolutionary rates with the test applied to the analysis, FMDVS, as other RNA viruses, are of limited genetic complexity and their population sizes are extremely large. Their evolution concerns complex, indeterminate mixtures of genomes rather than a single, determinate species.