Structural stability and bioapplicability assessment of hyaluronic acid-chitosan polyelectrolyte multilayers on titanium substrates
Structural stability and bioapplicability assessment of hyaluronic acid-chitosan polyelectrolyte multilayers on titanium substrates
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DOI：
10.1002/jbm.a.31854
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发表时间：
2008-12-15
影响因子：
4.9
通讯作者：
Kang, E. T.
中科院分区：
文献类型：
--
作者：
Chua, P. H.;Neoh, K. G.;Kang, E. T.
由于与植入物相关的细菌感染仍然是其失败的主要原因，本研究研究了使用含有透明质酸（HA）和壳聚糖（CH）的聚电解质多层膜（PEMS）来赋予钛（Ti）抗菌性能。采用逐层沉积法在Ti上沉积HA和CH。利用大肠杆菌和金黄色葡萄球菌对功能化钛底物的抑菌效果进行了评价。与原始Ti相比，经HA和CH PEMs功能化的Ti表面细菌数量均减少了10个数量级。研究了PEMs的化学成分对其结构稳定性和抗菌效果的影响。PEMs的化学交联赋予了更大的结构稳定性，即使在磷酸盐缓冲盐水中长时间浸泡后也能保持抗菌性能。采用MTT法评价PEMs对成骨细胞的细胞毒性。结果表明，功能化Ti底物的生物相容性和持久抗菌特性为减少植入物相关感染提供了巨大的潜力。(C) 2008 Wiley期刊有限公司[J] .中国生物医学工程学报，2016,31 (2):444 - 444
Since bacterial infections associated with implants remain a major cause of their failure, this study investigated the use of polyelectrolyte multilayers (PEMS) comprising hyaluronic acid (HA) and chitosan (CH) to confer antibacterial properties on titanium (Ti). HA and CH were deposited On Ti using the layer-by-layer deposition method. The antibacterial efficacy Of the functionalized Ti substrates was assessed using Escherichia coli and Staphylococcus aureus. The number of adherent bacteria oil Ti functionalized with HA and CH PEMs was Lip to all order of magnitude lower than that on the pristine Ti. The effects of chemical of the PEMs on the structural stability and antibacterial efficacy were investigated. The chemical crosslinking of the PEMs imparts greater Structural stability and preserves the antibacterial properties even after the prolonged immersion in phosphate-buffered saline. The cytotoxicity of the PEMs to osteoblasts was evaluated Using the MTT assay. The results showed that the biocompatible and long-lasting antibacterial nature of the functionalized Ti substrates offers great potential for reducing implant-associated infections. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 87A: 1061-1074, 2008