Expression of Eya1 in mouse taste buds
Expression of Eya1 in mouse taste buds
复制标题
Eya1在小鼠味蕾中的表达
DOI：
10.1007/s00441-020-03311-9
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发表时间：
2021
期刊：
影响因子：
3.6
通讯作者：
Hirota J
中科院分区：
文献类型：
--
作者：
Ohmoto M;Kitamoto S;Hirota J
味觉物质由口腔上皮中味蕾中的味觉受体细胞检测。单个味觉感受器细胞有助于唤起五种味觉品质之一：甜，鲜味，苦，酸和咸（钠）。它们每隔几周就会被局部上皮干细胞产生的新细胞所取代。POU转录因子Pou 2f 3（也称为Skn-1a）调节甜味、鲜味和苦味细胞的产生和分化。然而，最终分化为这些Pou 2f 3依赖性味觉受体细胞的分子机制仍然未知。为了鉴定调节这些味觉受体细胞分化的候选分子，我们使用甜味和苦味细胞的RNA序列数据搜索味觉受体类型特异性转录因子。没有发现在甜细胞中表达量高于在苦细胞中表达量的转录因子基因，Eya 1是唯一在苦细胞中表达量高于甜细胞的转录因子基因。原位杂交显示，Eya 1主要表达在苦味细胞，也在假定的未成熟/分化的味蕾细胞在轮廓和菌状乳头和软腭。Eya 1是调控苦味细胞生成和分化的候选分子。
Taste substances are detected by taste receptor cells in the taste buds in the oral epithelium. Individual taste receptor cells contribute to evoking one of the five taste qualities: sweet, umami, bitter, sour, and salty (sodium). They are continuously replaced every few weeks by new ones generated from local epithelial stem cells. A POU transcription factor, Pou2f3 (also known as Skn-1a), regulates the generation and differentiation of sweet, umami, and bitter cells. However, the molecular mechanisms underlying terminal differentiation into these Pou2f3-dependent taste receptor cells remain unknown. To identify the candidate molecules that regulate the differentiation of these taste receptor cells, we searched for taste receptor type-specific transcription factors using RNA-sequence data of sweet and bitter cells. No transcription factor gene showing higher expression in sweet cells than in bitter cells was found. Eyes absent 1 (Eya1) was identified as the only transcription factor gene showing higher expression in bitter cells than in sweet cells. In situ hybridization revealed that Eya1 was predominantly expressed in bitter cells and also in the putative immature/differentiating taste bud cells in circumvallate and fungiform papillae and soft palate. Eya1 is a candidate molecule that regulates the generation and differentiation of bitter cells.