In silico identification of the full complement of subtilase-encoding genes and characterization of the role of TaSBT1.7 in resistance against stripe rust in wheat
In silico identification of the full complement of subtilase-encoding genes and characterization of the role of TaSBT1.7 in resistance against stripe rust in wheat
复制标题
枯草杆菌酶编码基因的完整互补体的计算机鉴定和 TaSBT1.7 在小麦抗条锈病中的作用的表征
DOI：
10.1094/phyto-05-20-0176-r
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Shunyuan Xiao
中科院分区：
文献类型：
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作者：
Yuheng Yang;Fengfeng Zhang;Tianyu Zhou;Anfei Fang;Yang Yu;chaowei Bi;Shunyuan Xiao
植物枯草酶（sbt）或枯草酶样蛋白酶包括一个非常多样化的丝氨酸肽酶家族，参与广泛的生物功能。尽管近年来有越来越多的证据表明SBTs在植物免疫中的作用，但对小麦（Triticum aestivum）的SBTs （tasbt）知之甚少。本研究利用最新版本的参考基因组序列2.0从面包小麦中鉴定出255TaSBTgenes。根据推导出的蛋白序列构建的系统发育树，SBT家族可分为从TaSBT1到TaSBT5五个支系。在硅蛋白结构域分析中发现，TaSBT家族存在相当大的序列多样化，加上局部聚集的基因分布，表明TaSBT基因经历了广泛的功能多样化。在被生物因子改变表达的setasbtt基因中，tasbt1.7被发现在小麦叶片中被几丁质和flg22激发子诱导，以及6种被检测的病原体诱导，这意味着tasbt1.7在植物防御中起作用。烟叶中tasbt1.7的短暂过表达导致坏死细胞死亡。此外，使用大麦条纹花叶病毒诱导的基因沉默来敲除小麦中的tasbt1.7，会损害小麦对条纹锈病的过敏反应和抗性。小麦条锈病的病原小麦锈菌。综上所述，本研究确定了小麦sbt1.7基因的完整补体，并为其中一个特定成员TaSBT1.7在小麦与条锈病病原的不亲和互作中发挥积极作用提供了证据。
Plant subtilases (SBTs) or subtilisin-like proteases comprise a very diverse family of serine peptidases that participates in a broad spectrum of biological functions. Despite increasing evidence for roles of SBTs in plant immunity in recent years, little is known about wheat (Triticum aestivum) SBTs (TaSBTs). Here, we identified 255TaSBTgenes from bread wheat using the latest version 2.0 of the reference genome sequence. The SBT family can be grouped into five clades, from TaSBT1 to TaSBT5, based on a phylogenetic tree constructed with deduced protein sequences. In silico protein-domain analysis revealed the existence of considerable sequence diversification of the TaSBT family which, together with the local clustered gene distribution, suggests thatTaSBTgenes have undergone extensive functional diversification. Among thoseTaSBTgenes whose expression was altered by biotic factors,TaSBT1.7was found to be induced in wheat leaves by chitin and flg22 elicitors, as well as six examined pathogens, implying a role forTaSBT1.7in plant defense. Transient overexpression ofTaSBT1.7inNicotiana benthamianaleaves resulted in necrotic cell death. Moreover, knocking downTaSBT1.7in wheat using barley stripe mosaic virus-induced gene silencing compromised the hypersensitive response and resistance againstPuccinia striiformisf. sp.tritici, the causal agent of wheat stripe rust. Taken together, this study defined the full complement of wheatSBTgenes and provided evidence for a positive role of one particular member,TaSBT1.7, in the incompatible interaction between wheat and a stripe rust pathogen.