Diagnostic Significance of Overexpression of Golgi Membrane Protein 1 in Prostate Cancer
Diagnostic Significance of Overexpression of Golgi Membrane Protein 1 in Prostate Cancer
复制标题
高尔基体膜蛋白1过表达在前列腺癌中的诊断意义
DOI：
10.1016/j.urology.2012.06.017
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发表时间：
2012-10-01
期刊：
影响因子：
2.1
通讯作者：
Chen, Gang
中科院分区：
文献类型：
--
作者：
Li, Wenzhi;Wang, Xiang;Chen, Gang
目的探讨高尔基体膜蛋白1（Golgi membrane protein 1，GOLM 1）在前列腺癌组织中的表达及其诊断意义。分别通过定量实时聚合酶链反应和蛋白质印迹分析来测定前列腺癌细胞中GOLM 1在mRNA和蛋白质水平的表达。结果免疫荧光结果显示GOLM 1蛋白定位于DU 145细胞的顺式高尔基体（cis-Golgi）。GOLM 1转录物和蛋白质在多种前列腺癌细胞系（DU 145、22 RV 1、PC-3和LNCaP）中过表达。组织芯片免疫组化结果显示，GOLM 1蛋白偶见于正常前列腺和良性前列腺增生，而在前列腺癌中主要见于肿瘤细胞的胞浆。GOLM 1蛋白在前列腺癌组织中呈强阳性表达，与正常前列腺组织和前列腺增生组织相比，差异有统计学意义（P < .05）。GOLM 1蛋白在前列腺癌组织中的表达与前列腺癌组织学分级、病理分期无显著性差异（P > 0.05）。结论GOLM 1蛋白在前列腺癌组织中的表达明显高于正常前列腺组织和前列腺增生组织。这些发现可能表明GOLM 1在前列腺癌的诊断或治疗中是有用的。然而，GOLM 1过表达与疾病分期和分级无关。泌尿学80：952.e1-952.e7，2012年。(C)2012 Elsevier Inc.
OBJECTIVE To investigate the diagnostic significance of Golgi membrane protein 1 (GOLM1) expression in prostate cancer.METHODS The localization of GOLM1 in prostate cancer cells was detected by immunofluorescence. The GOLM1 expression in prostate cancer cells at the mRNA and protein level was determined by quantitative real-time polymerase chain reaction and Western blot analysis, respectively. A prostate cancer tissue microarray was used to analyze GOLM1 protein expression by immunohistochemistry.RESULTS The immunofluorescence results demonstrated that GOLM1 was located at the cis-Golgi in the DU145 cells. GOLM1 transcripts and protein were overexpressed in a wide variety of prostate cancer cell lines (DU145, 22RV1, PC-3, and LNCaP). Tissue microarray immunohistochemistry demonstrated that GOLM1 protein staining was occasionally found in the normal prostate gland and benign prostatic hyperplasia, whereas that in prostate cancer was predominantly observed in the cytoplasm of tumor cells. GOLM1 protein was strongly expressed in prostate cancer tissues, and there was a significant difference compared with the normal prostate and benign prostatic hyperplasia cases (P < .05). There were no significant differences between GOLM1 overexpression and pathologic variables of prostate cancer, including histologic grade and pathologic stage (P > .05).CONCLUSION Our results suggest that GOLM1 protein is significantly expressed in prostate cancer in comparison with the normal prostate gland and benign prostatic hyperplasia. These findings may suggest that GOLM1 is useful in the diagnosis or therapy of prostate cancer. However, GOLM1 overexpression is not associated with disease stage and grade. UROLOGY 80: 952.e1-952.e7, 2012. (C) 2012 Elsevier Inc.