Orally administered conjugated linoleic acid ameliorates allergic dermatitis induced by repeated applications of oxazolone in mice
Orally administered conjugated linoleic acid ameliorates allergic dermatitis induced by repeated applications of oxazolone in mice
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口服共轭亚油酸可改善小鼠反复使用恶唑酮诱发的过敏性皮炎
DOI：
10.1111/asj.12603
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发表时间：
2016
影响因子：
2
通讯作者：
Kawahara S.
中科院分区：
文献类型：
--
作者：
Nakanishi T.;Tokunaga Y.;Yamasaki M.;Erickson L.;Kawahara S.
共轭亚油酸（CLA）是动物产品的成分之一，具有潜在的健康益处，如抗癌和抗肥胖作用。在这项研究中，我们研究了共轭亚油酸的免疫调节作用，使用小鼠过敏性皮炎模型。小鼠经口给予含有等量9 c，11 t-CLA和10 t，12 c-CLA的CLA混合物或高亚油酸红花油，并通过重复局部应用恶唑酮在耳上诱导过敏性皮炎。口服CLA混合物而非高亚油酸红花油可减轻过敏性皮炎的症状，包括耳重和临床评分。这种作用与耳白细胞介素-4（IL-4）和血浆免疫球蛋白E水平降低有关。通过体外细胞因子产生测定比较CLA异构体的免疫调节作用。结果表明，动物产品中最主要的异构体9 c，11 t-CLA显著抑制小鼠脾细胞产生IL-4和干扰素-γ，其效力与10 t，12 c-CLA相似。这些结果表明，CLA，动物产品的组成部分，有一个潜在的有益的作用，改善过敏性皮炎。
Conjugated linoleic acid (CLA) is one of the constituents of animal products with possible health benefits such as anti‐carcinogenic and anti‐obesity effects. In this study, we investigated the immunomodulatory effects of CLA using a mouse model of allergic dermatitis. Mice were orally administered either a CLA mixture containing equal amounts of 9c, 11 t‐CLA and 10 t, 12c‐CLA, or high linoleic acid safflower oil, and allergic dermatitis was induced on the ear by repeated topical applications of oxazolone. Oral administration of the CLA mixture but not the high linoleic safflower oil attenuated the symptoms of allergic dermatitis in both ear weights and clinical scores. This effect was associated with decreased levels of ear interleukin‐4 (IL‐4) and plasma immunoglobulin E. The immunomodulatory effects of the CLA isomers were compared by anin vitrocytokine production assay. The results showed that 9c, 11 t‐CLA, the most predominant isomer in animal products, significantly inhibited IL‐4 and interferon‐γ production from mouse splenocytes with similar potency to 10 t, 12c‐CLA. These findings suggest that CLA, a constituent of animal products, has a potentially beneficial effect for amelioration of allergic dermatitis.