Immunoprotection against influenza virus H9N2 by the oral administration of recombinant Lactobacillus plantarumNC8 expressing hemagglutinin in BALB/c mice
Immunoprotection against influenza virus H9N2 by the oral administration of recombinant Lactobacillus plantarumNC8 expressing hemagglutinin in BALB/c mice
复制标题
BALB/c小鼠口服表达血凝素的重组植物乳杆菌NC8对流感病毒H9N2的免疫保护作用
DOI：
10.1016/j.virol.2014.07.011
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发表时间：
2014-09-01
期刊：
影响因子：
3.7
通讯作者：
Li，Yu
中科院分区：
文献类型：
--
作者：
Shi，Shao-Hua;Yang，Wen-Tao;Li，Yu
H9 N2禽流感病毒（AIV）由于其在家禽业中造成严重经济损失的作用而日益受到关注。禽流感病毒传播给哺乳动物，包括猪和人，加快了制定预防战略的努力。为研制有效的H9 N2亚型禽流感口服疫苗，本研究构建了表达H9 N2亚型禽流感病毒血凝素（HA）基因的重组植物乳杆菌NC 8株。用重组NC 8-pSIP 409-HA株经口免疫小鼠，产生sIgA、IgG和HI抗体，并诱导CD 8 +T细胞免疫应答。最重要的是，口服给药对小鼠适应性H9 N2病毒的攻击产生了完全的保护作用。这些结果表明重组NC 8-pSIP 409-HA更有效地诱导粘膜、体液和细胞免疫反应。因此，L. plantarumNC 8-pSIP 409-HA有望成为H9 N2亚型禽流感口服疫苗候选者。
The H9N2 avian influenza virus (AIV) has become increasingly concerning due to its role in severe economic losses in the poultry industry. Transmission of AIV to mammals, including pigs and humans, has accelerated efforts to devise preventive strategies. To develop an effective oral vaccine against H9N2 AIV, a recombinantLactobacillus plantarumNC8 strain expressing the hemagglutinin (HA) gene of H9N2 AIV was constructed in this study. Mice were orally immunized with the recombinant NC8-pSIP409-HA strain, and sIgA, IgG and HI antibodies were produced by the NC8-pSIP409-HA strain, which also induced CD8+T cell immune responses. Most importantly, oral administration produced complete protection against challenge with mouse-adapted H9N2 virus. These results indicate that the recombinant NC8-pSIP409-HA was more effective at inducing the mucosal, humoral and cellular immune responses. Therefore,L. plantarumNC8-pSIP409-HA could become a promising oral vaccine candidate against H9N2 AIV.