Disinfection of Pseudomonas aeruginosa biofilm contaminated tube lumens with ultraviolet C light emitting diodes
Disinfection of Pseudomonas aeruginosa biofilm contaminated tube lumens with ultraviolet C light emitting diodes
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DOI：
10.1080/08927010903191353
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发表时间：
2010-01-01
期刊：
影响因子：
2.7
通讯作者：
Gregersen, Annette
中科院分区：
文献类型：
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作者：
Bak, Jimmy;Ladefoged, Soren D.;Gregersen, Annette
长期导管上的细菌生物膜是感染的主要来源。在早期的研究中显示，暴露于紫外线C（UVC - 265 nm）光可显著减少体外处理的患者导尿管上的细菌数量。然而，为了达到99.9%的消毒率，应使用非常大的剂量（长时间处理）。其主要原因是成熟生物膜中除细胞外还含有吸收和散射颗粒，使生物膜不透明。研究了UVC发光二极管（LED）用于生物膜污染的导管类管道消毒的潜力。结果表明，UVC光传播是可能的，通过聚四氟乙烯和导管（硅树脂）。二极管的消毒效率被证明对人工污染的铜绿假单胞菌生物膜管。将管连接到流动系统，并在3天的时间内产生生物膜。长度为10 cm（Teflon，硅胶）和20 cm（Teflon）的管被污染。用于对照和用于UVC处理的管被平行污染。从管的总内表面取样生物膜。对照样品上的菌落计数范围为5 x 10（5）-1.3 x 10（9）CFU ml（-1），消毒率范围为96- 100%。所施加的UVC剂量对应于15和300分钟之间的处理时间。在暴露30分钟的10 cm Teflon管中获得消毒（100
Bacterial biofilms on long-term catheters are a major source of infection. Exposure to ultraviolet C (UVC - 265 nm) light was shown in an earlier study to reduce the number of bacteria substantially on ex vivo treated urinary patient catheters. Very large doses ( long treatment times) should, however, be applied to obtain 99.9% disinfection rates. The major reason was that besides cells the mature biofilm contained absorbing and scattering particulates, which made the biofilm opaque. The potential of UVC light emitting diodes ( LED) for disinfection purposes in catheter-like tubes contaminated with biofilm was investigated. It was shown that UVC light propagation was possible through both Teflon and catheter tubes ( silicone). The disinfection efficiency of the diodes was demonstrated on tubes contaminated artificially with a Pseudomonas aeruginosa biofilm. The tubes were connected to a flow system and biofilms were produced during a 3 day period. Tubes in lengths of 10 ( Teflon, silicone) and 20 cm ( Teflon) were contaminated. Tubes for control and for UVC treatment were contaminated in parallel. Biofilms were sampled from the total inner surface of the tubes. Colony counts on the control samples were in the range of 5 x 10(5)-1.3 x 10(9) CFU ml(-1), with disinfection rates in the range 96-100%. The applied UVC doses corresponded to treatment times between 15 and 300 min. Disinfection (100%) was obtained in 10 cm Teflon tubes exposed for 30 min (detection limit