Cellular proteomic analysis of porcine circovirus type 2 and classical swine fever virus coinfection in porcine kidney-15 cells using isobaric tags for relative and absolute quantitation-coupled LC-MS/MS
Cellular proteomic analysis of porcine circovirus type 2 and classical swine fever virus coinfection in porcine kidney-15 cells using isobaric tags for relative and absolute quantitation-coupled LC-MS/MS
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使用同量异位标签进行相对和绝对定量耦合 LC-MS/MS 对猪肾 15 细胞中猪圆环病毒 2 型和猪瘟病毒共感染进行细胞蛋白质组分析
DOI：
10.1002/elps.201600541
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发表时间：
2017-05-01
期刊：
影响因子：
2.9
通讯作者：
Zhou, Jiyong
中科院分区：
文献类型：
--
作者：
Zhou, Niu;Fan, Chunmei;Zhou, Jiyong
病毒在宿主体内的混合感染或重复感染已经引起了公共卫生问题和养殖业的巨大经济损失。病毒共感染对细胞蛋白质丰度的影响是病毒致病的关键。基于本实验室建立的猪圆环病毒2型（PCV 2）和猪瘟病毒（CSFV）共感染模型，采用同量异序标签相对和绝对定量（iTRAQ）联用的LC-MS/MS蛋白质组分析技术，研究了PCV 2和CSFV共感染宿主细胞的反应。在三个独立的质谱分析中总共鉴定了3932种蛋白质。与未感染细胞相比，304个蛋白质增加（倍数变化>1.2），198个蛋白质减少（倍数变化
Viral coinfection or superinfection in host has caused public health concern and huge economic losses of farming industry. The influence of viral coinfection on cellular protein abundance is essential for viral pathogenesis. Based on a coinfection model for porcine circovirus type 2 (PCV2) and classical swine fever virus (CSFV) developed previously by our laboratory, isobaric tags for relative and absolute quantitation (iTRAQ)-coupled LC-MS/MS proteomic profiling was performed to explore the host cell responses to PCV2-CSFV coinfection. Totally, 3932 proteins were identified in three independent mass spectrometry analyses. Compared with uninfected cells, 304 proteins increased (fold change >1.2) and 198 decreased (fold change