Infection with cytomegalovirus during pregnancy: specific IgM antibodies as a marker of recent primary infection.
Infection with cytomegalovirus during pregnancy: specific IgM antibodies as a marker of recent primary infection.
复制标题
DOI：
10.1093/infdis/145.2.647
复制
发表时间：
1982-05
期刊：
影响因子：
--
通讯作者：
P. Griffiths;S. Stagno;R. F. Pass;Richard Smith;C. Alford
中科院分区：
文献类型：
--
作者：
P. Griffiths;S. Stagno;R. F. Pass;Richard Smith;C. Alford
特异性IgM抗体持续长达4个月的放射免疫测定法（RIA）中检测到16（55%）的29名妇女的血清原发感染由于巨细胞病毒（CMV）。IgM的RIA检测原发性感染6（86%）的7个血清中获得的4个月内的血清转换。与此相反，IgM抗体从来没有检测到RIA在104例血清样本，18名妇女反复感染由于CMV，无论是否发生宫内传播的病毒。特异性IgM抗体也被检测到在最早的样品在怀孕期间的血清从三个（14%）的21名妇女的CMV感染的类型是未知的，但谁已经交付的先天性感染的婴儿。所有这些结果表明，在怀孕的第一个三个月与CMV的原发感染可以通过测试一个单一的血清样品，用RIA的IgM抗体诊断。尝试通过免疫荧光法测量IgM抗体，假阳性反应的频率很高（19/104 [18%]）。
Specific IgM antibodies that persisted for up to four months were detected by radioimmunoassay (RIA) in the sera of 16 (55%) of 29 women with primary infections due to cytomegalovirus (CMV). The RIA for IgM detected primary infections in six (86%) of seven sera obtained within four months of seroconversion. In contrast, IgM antibodies were never detected by RIA in 104 serum samples from 18 women with recurrent infections due to CMV, irrespective of whether intrauterine transmission of virus had occurred. Specific IgM antibodies were also detected in the earliest samples during pregnancy of serum from three (14%) of 21 women whose type of infection with CMV was unknown but who had been delivered of congenitally infected infants. All of these results show that primary infection with CMV in the first trimester of pregnancy can be diagnosed by testing a single serum sample by RIA for IgM antibodies. Attempts to measure IgM antibodies by immunofluorescence gave a high frequency (19 [18%] of 104) of false-positive reactions.