Effects of Temperature and Humidity on Lychee (Litchi chinensis Sonn.) Pollen Germination during Anther Dehiscence
Effects of Temperature and Humidity on Lychee (Litchi chinensis Sonn.) Pollen Germination during Anther Dehiscence
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温湿度对荔枝花药开裂期间花粉萌发的影响
DOI：
10.11248/jsta.61.62
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Higuchi Hirokazu
中科院分区：
文献类型：
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作者：
Matsuda Hiroshi;Higuchi Hirokazu
我们确定了开花期间导致荔枝花粉高萌发的温度和湿度条件。在中国南方栽培品种'Tai So'、'Souey Tung'、'Floridian'Bengal '和' Thai 'Chakrapat'的花药开裂前采集雄花，然后在不同温度（15-30 ℃）和相对湿度[RH]（10-75%）组合下培养24小时。培养后，记录花药开裂的百分比，然后测试花粉萌发。在所有品种中，开裂增加在较高的温度或较低的湿度。在≥ 20 ℃和RH<60%条件下，24 h内开裂率超过90%，但“小桐”的开裂率一般低于80%。“孟加拉”在20 ℃、相对湿度为50%左右时花药开裂，萌发率最高，达50%，随温度的升高和降低，萌发率有下降的趋势。随着湿度的增加，种子萌发率有下降的趋势，且在≥ 25 ℃时，这种趋势最为显著. Chakrapat'发芽率也最高（约60%），在20 ℃和约50%RH。Chakrapat'在30 ℃发芽相对较好。相对湿度> 30%对种子萌发影响不大。在20 ℃、相对湿度为150%时，‘小桐’和‘大苏’的发芽率最高，分别为1035%和1030%，但发芽率较低。与Chakrapat相似，‘大苏’的发芽率在较高温度下略有下降，相对湿度> 30%对发芽率的影响不大。花粉管伸长在20 ℃、相对湿度≥ 50%RH时最好，在较高温度或较低湿度时有下降趋势。荔枝花药开裂的最适温度/湿度条件为20 ℃/± 50%RH，与品种无关。
We determined the temperature and humidity conditions during flowering that result in high germination of lychee pollen. Staminate flowers were gathered before anther dehiscence from the South Chinese cultivars ‘Tai So,’‘Souey Tung,’Floridian ‘Bengal,’and Thai ‘Chakrapat,’and then incubated for 24 h at various temperature (15–30 C) and relative humidity [RH](10–75%) combinations. After incubation, the percentage of dehisced anthers was recorded, and then pollen germination was tested. In all cultivars, dehiscence increased at higher temperature or lower humidity. The dehiscence exceeded 90% within 24 h at≥ 20 C and RH< 60%, except for ‘Souey Tung,’for which it was generally below 80%. Germination of ‘Bengal’was highest (≈ 50%) when the anthers dehisced at 20 C and≈ 50% RH, and tended to decrease at higher and lower temperatures. The germination tended to decrease as humidity increased, and this tendency was remarkable at≥ 25 C.‘Chakrapat’germination was also highest (≈ 60%) at 20 C and≈ 50% RH.‘Chakrapat’germinated relatively well at 30 C. Relative humidity> 30% affected germination slightly. Similarly,‘Souey Tung’and ‘Tai So’had the highest germination (≈ 35% and≈ 30%, respectively) at 20 C and≈ 50% RH, although the germination was low. Similar to ‘Chakrapat,’‘Tai So’germination decreased slightly at higher temperatures, and was affected little by RH> 30%. Pollen-tube elongation was also best at 20 C and≈ 50% RH, and tended to decrease at higher temperatures or lower humidity. The optimal temperature/humidity conditions for lychee anther dehiscence were 20 C/≈ 50% RH, regardless of the cultivar.
温度和培养基成分对“Bengal”和“Chakrapat”荔枝（Litchi chinensis Sonn.）花粉离体萌发的影响
DOI：
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发表时间：
2013
期刊：
Trop. Agr. Develop.
影响因子：
--
作者：
Naoko KOZAI;Orwintinee CHUSRI;Theerawut CHUTINANTHAKUN;Sombat TONGTAO;Hirokazu HIGUCHI;and Tatsushi OGATA;Hiroshi MATSUDA and Hirokazu HIGUCHI
通讯作者：
Hiroshi MATSUDA and Hirokazu HIGUCHI