A versatile shear and compression apparatus for mechanical stimulation of tissue culture explants
A versatile shear and compression apparatus for mechanical stimulation of tissue culture explants
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DOI：
10.1016/s0021-9290(00)00100-7
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发表时间：
2000-11-01
影响因子：
2.4
通讯作者：
Grodzinsky, AJ
中科院分区：
文献类型：
--
作者：
Frank, EH;Jin, M;Grodzinsky, AJ
我们已经开发了一个孵化器，双轴组织加载装置能够应用轴向变形小到1妈妈和正弦旋转小到0.01度。轴向分辨率为50纳米，适用于低至10微米的正弦波（或基于1毫米厚度的1%）或大至100微米的旋转分辨率为0.0005度。该机器足够小（30厘米高x 25厘米x 20厘米），可以放置在标准培养箱中进行长期组织培养负载研究。在本文描述的代谢研究中，应用正弦宏观剪切变形对关节软骨外植体的影响导致蛋白聚糖和蛋白质的合成（s -35-硫酸盐和h -3-脯氨酸的摄取）比在相同的静态偏移压缩下保持的对照显著增加。(C) 2000 Elsevier Science Ltd.版权所有。
We have developed an incubator housed, biaxial-tissue-loading device capable of applying axial deformations as small as 1 mum and sinusoidal rotations as small as 0.01 degrees. Axial resolution is 50 nm for applying sinewaves as low as 10 mum (or 1% based on a 1 mm thickness) or as large as 100 mum Rotational resolution is 0.0005 degrees. The machine is small enough (30 cm high x 25 cm x 20 cm) to be placed in a standard incubator for long-term tissue culture loading studies. In metabolic studies described here, application of sinusoidal macroscopic shear deformation to articular cartilage explants resulted in a significant increase in the synthesis of proteoglycan and proteins (uptake of S-35-sulfate and H-3-proline) over controls held at the same static offset compression. (C) 2000 Elsevier Science Ltd. All rights reserved.