Enhanced biological activity of polymeric osteopontin
Enhanced biological activity of polymeric osteopontin
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DOI：
10.1016/j.febslet.2007.05.018
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发表时间：
2007-06-12
期刊：
影响因子：
3.5
通讯作者：
Yokosaki, Yasuyuki
中科院分区：
文献类型：
--
作者：
Higashikawa, Fumiko;Eboshida, Akira;Yokosaki, Yasuyuki
骨桥蛋白是一种多功能糖蛋白，主要通过整合素介导免疫调节、炎症反应、组织矿化和组织重塑。转氨酶2通过异肽键选择性地交联蛋白质。骨桥蛋白是这种酶的底物之一，并经历聚合;然而，这种聚合的生物学意义仍然未知。使用重组骨桥蛋白与纯化的转氨酶2聚合，我们研究了细胞粘附，扩散，局部接触形成，和迁移的SW 480或HUVE细胞。所有这些细胞的行为显着增强与聚合骨桥蛋白。这些细胞功能的增强意味着骨桥蛋白的聚合可能调节生理和病理功能。(c)2007年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Osteopontin is a multifunctional glycoprotein with roles in immunomodulation, inflammatory response, tissue mineralization, and tissue remodeling, which are mediated primarily through integrins. Transglutaminase 2 selectively cross-links proteins by isopeptide bonding. Osteopontin is one of the substrates of this enzyme and undergoes polymerization; however, the biological meaning of this polymerization remains unknown. Using recombinant osteopontin polymerized with purified transglutaminase 2, we examined cell adhesion, spreading, focal contact formation, and migration of SW480 or HUVE cells. All of these cellular behaviors were dramatically enhanced with polymeric osteopontin. These enhancements of cellular functions imply that polymerization might modulate physiological and pathological functions of osteopontin. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.