Azorhizobium caulinodans ORS571 colonizes the xylem of Arabidopsis thaliana
Azorhizobium caulinodans ORS571 colonizes the xylem of Arabidopsis thaliana
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DOI：
10.1094/mpmi.2001.14.1.93
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发表时间：
2001-01-01
影响因子：
3.5
通讯作者：
Cocking, EC
中科院分区：
文献类型：
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作者：
Stone, PJ;O'Callaghan, KJ;Cocking, EC
利用改良的拟南芥无菌生长条件，研究了茎固氮瘤菌ORS571对拟南芥木质部的定植情况。当接种带有LacZ报告基因的ORS571(PXLGD4)的幼苗时，几乎所有的植株都在侧根裂缝(LRC)处出现了ORS571定植的蓝色区域。黄酮类化合物柚皮苷元和甘草次皂苷元对ORS571对LRC的定植有明显的促进作用。用组织化学方法在接种植物完整根的木质部中观察到ORS571(PXLGD4)细菌的蓝色条带。对接种沙冬青的初生根和侧根切片进行详细的显微分析，证实了木质部的定植。在缺乏结瘤因子的ORS571NODC突变体中，木质部也发生了定殖。接种ORS571(PXLGD4)或ORS571：：NODC(PXLGD4)的植株，在接种或不接种柚皮苷元的情况下，其木质部定殖率和平均每株木质部的长度没有显著差异。
Improved conditions were used for the aseptic growth of Arabidopsis thaliana to investigate whether xylem colonization of A. thaliana by Azorhizobium caulinodans ORS571 might occur. When seedlings were inoculated with ORS571 (pXLGD4) tagged with the lacZ reporter gene, nearly all of the plants showed blue regions of ORS571 colonization at lateral root cracks (LRC). The flavonoids naringenin and liquiritigenin significantly stimulated colonization of LRC by ORS571. Blue bands of ORS571 (pXLGD4) bacteria were observed histochemically in the xylem of intact roots of inoculated plants. Detailed microscopic analysis of sections of primary and lateral roots from inoculated A. thaliana confirmed xylem colonization. Xylem colonization also occurred with an ORS571 nodC mutant deficient in nodulation factors. There was no significant difference in the percentage of plants with xylem colonization or in the mean length of xylem colonized per plant between plants inoculated with either ORS571 (pXLGD4) or ORS571::nodC (pXLGD4), with or without naringenin.