Properties of ECHO types 22, 23 and 24 viruses
Properties of ECHO types 22, 23 and 24 viruses
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DOI：
10.1007/bf01241688
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发表时间：
1961-01-01
期刊：
影响因子：
--
通讯作者：
SABIN, ALBERT B.
中科院分区：
文献类型：
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作者：
WIGAND, REINHARD;SABIN, ALBERT B.
目前分类为ECHO 22、23和24的病毒是从腹泻婴儿直肠拭子的猴肾组织培养中分离出来的。回声22和回声23病毒的细胞致病作用通常局限于培养物的外周部分。这些病毒的传代和适应遇到了困难。eec24病毒影响整个培养物。在猴肾单分子层上，ECHO 22和23病毒产生圆形和规则斑块，ECHO 24病毒产生不规则斑块。除一株ECHO 24外，所有病毒在空斑瓶琼脂培养基下的滴度都明显高于用液体培养基培养的滴度。通过空斑纯化和纯化病毒制备的兔抗血清对原病毒和纯化病毒的抗血清检测，表明各菌株的抗原性均一致。它们对新生小鼠无致病性，对棘内和脑内接种的食蟹猴无明确的神经致病性。在中和试验中，它们与1 ~ 21型ECHO病毒、1 ~ 3型脊髓灰质炎病毒、柯萨奇A9病毒、B1到B5病毒以及彼此之间没有交叉反应。与腺病毒无血清学或其他关系。回声22和23病毒可被人γ球蛋白中和，回声24病毒不能被人血清中和。3株ECHO - 22病毒的抗原性存在差异。摘要作者：作者
The viruses now classified as ECHO 22, 23, and 24 were isolated in monkey kidney tissue culture from rectal swabs of infants with diarrhea. The cytopathogenic effect of ECHO 22 and 23 viruses is often confined to the peripheral part of the culture. Difficulties were encountered with passage and adaptation of these viruses. ECHO 24 virus affects the whole culture. On monkey kidney monolayers ECHO 22 and 23 viruses produce round and regular plaques, and ECHO 24 irregular plaques. All viruses, except one strain of ECHO 24, yielded considerably higher titers under agar in plaque bottles than in tube cultures with fluid medium. The individual strains were shown to be antigenically homogeneous by means of plaque purification and testing of rabbit anti-sera prepared with purified virus against both original and purified virus. They were not pathogenic for newborn mice and exhibited no definite neuropathogenicity for cynomolgus monkeys after intraspinal and intra-cerebral inoculation. In neutralization tests they showed no cross-reactions with ECHO virus types 1 to 21, polioviruses 1 to 3, Coxsackie A9, B1 through B5 virus, and among each other. No serologic or other relation to adenovirus was found. ECHO 22 and 23 viruses were neutralized by human gamma globulin, while ECHO 24 virus was not, but was neutralized by individual human sera. Antigenic differences were shown among 3 strains of ECHO 22 virus. || ABSTRACT AUTHORS: Authors