Noncontact specular microscopy of human lens epithelium.
Noncontact specular microscopy of human lens epithelium.
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发表时间：
2000-02
影响因子：
4.4
通讯作者：
M. Balaram;W. Tung;J. Kuszak;M. Ayaki;T. Shinohara;L. Chylack
中科院分区：
文献类型：
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作者：
M. Balaram;W. Tung;J. Kuszak;M. Ayaki;T. Shinohara;L. Chylack
目的：获得年龄相关性白内障（ARC）患者和非年龄相关性白内障患者的透镜上皮细胞（LEC）的活体镜面反射图像;识别描述这些复杂图像的各个方面的特征;开发特征尺度以量化每个特征的严重程度;并研究这些特征与LEC计数，年龄，ARC中透镜上皮的透镜混浊分类系统III（LOCS III）分类和显微镜特征。方法152例患者接受眼科检查和LOCS III白内障分类。使用改良的非接触式角膜镜面反射显微镜（SML-2; Konan，Hyogo，Japan）捕获镜片的镜面反射图像。增强图像以掩蔽的方式分级，镜面图像中四个特征（“柱状组织”、“线性沟槽”、“蓬松云”和“黑洞”）的存在或不存在以及严重程度以四个等级分级。采用组内相关的广义线性模型确定年龄、性别、LOCS III级、细胞计数和特征分级之间的相关性的统计学意义。本文对29例晶状体囊破裂标本进行了相关的光镜和电镜观察。结果LEC密度随着年龄的增长而下降，并与膨胀云的标量等级以及黑洞的大小和数量呈负相关。柱状组织的标量分级与后囊下和核性白内障的严重程度呈负相关，这是与ARC的LOCS III级相关的唯一特征。平均细胞计数与LOCS III级之间无统计学显著相关性。结论角膜内皮显微镜可获得良好的晶状体上皮细胞的活体角膜内皮图像，并对图像中与显微镜下所见相关的特征进行分类，估计活体晶状体上皮细胞的密度。
PURPOSE To obtain in vivo specular images of human lens epithelial cells (LECs) from persons with or without age-related cataract (ARC); to identify features that describe individual aspects of these complex images; to develop feature scales to quantify the severity of each feature; and to study the association of these features with LEC count, age, Lens Opacity Classification System III (LOCS III) classifications and microscopic features of lens epithelium in ARC. METHODS One hundred fifty-two individuals underwent ophthalmic examinations and LOCS III cataract classifications. Specular images of lenses were captured using a modified noncontact corneal specular microscope (SML-2; Konan, Hyogo, Japan). Enhanced images were graded in a masked fashion, and the presence or absence and severity of each of four features in the specular image ("columnar organization," "linear furrows," "puffy clouds," and "black holes") was graded on a four-step scale. The generalized linear model with intraclass correlation was used to ascertain the statistical significance of associations between age, sex, LOCS III grade, cell count, and feature grade. Capsulorrhexis specimens from 29 patients were studied with correlative light and electron microscopy. RESULTS LEC density declined with age and was inversely correlated with the scalar grade for puffy clouds and for the size and number of black holes. The scalar grade for columnar organization was inversely associated with the severity of posterior subcapsular and nuclear cataracts, which was the only feature associated with the LOCS III grade of ARC. No statistically significant associations were found between average cell count and LOCS III grade. CONCLUSIONS With the use of the corneal specular microscope excellent in vivo specular images of the LECs were obtained, the features in these images that correlated well with microscopic findings were classified, and cell density in vivo was estimated.