New approaches for quantifying hyaluronic acid in pharmaceutical semisolid formulations using HPLC and CZE
New approaches for quantifying hyaluronic acid in pharmaceutical semisolid formulations using HPLC and CZE
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DOI：
10.1016/s0731-7085(02)00329-1
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发表时间：
2002-11-07
影响因子：
3.4
通讯作者：
Neubert, RHH
中科院分区：
文献类型：
--
作者：
Alkrad, JA;Merstani, Y;Neubert, RHH
使用HPLC-UV-检测器定量药物制剂中的HA，并在用浓H2SO 4消化后进行荧光定量。使用直接和间接方法通过毛细管区带电泳（CZE）定量完整HA。将结果与Bitter等人建立的咔唑反应（Anal. 4（1997）330）和Plazer等人（J. Pharm. Biomed. Anal. 21（1997）491）关于检测限、线性、再现性和简单性。目前的结果表明，使用HPLC和CZE的研究导致了相当大的改善的检测限[0.3 ng/ml（HPLCl），1 μ g/ml（HPLC 2）和5 μ g/ml（CE-D1）]相比，其他方法（10 μ g/ml）。(C)2002 Elsevier Science B. V.保留所有权利。
HA was quantified in pharmaceutical formulations using HPLC-UV-detector and spectrophotometrically after digestion with concentrated H2SO4. Intact HA was quantified by capillary zone electrophoresis (CZE) using direct and indirect methods. The results were compared with the carbazole reaction established by Bitter et al. (Anal. Biochem. 4 (1997) 330) and with established method from Plazer et al. (J. Pharm. Biomed. Anal. 21 (1997) 491) regarding detection limits, linearity, reproducibility and simplicity. The present results show that the investigation using HPLC and CZE led to a considerable improvement of the detection limit [0.3 ng/ml (HPLCl), 1 mug/ml (HPLC2) and 5 mug/ml (CE-D1)] compared with other methods (10 mug/ml). (C) 2002 Elsevier Science B.V. All rights reserved.