[Tissue-engineered models of the nervous system].
[Tissue-engineered models of the nervous system].
复制标题
[神经系统的组织工程模型]。
DOI：
10.1051/medsci/2009253288
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
F. Berthod
中科院分区：
文献类型：
--
作者：
Marie‐Michèle Beaulieu;P. Tremblay;F. Berthod
神经系统是非常复杂的，并暴露于各种创伤和退行性疾病，仍然难以治疗。为了促进其研究，在体外模型，通过培养神经元和神经胶质细胞在单层培养，或通过脑或脊髓切片的器官型培养。这些体外模型过去和现在仍然对神经科学的进步非常有帮助。然而，它们对于某些研究来说，要么过于简单，要么过于复杂。组织工程在神经科学中的应用提供了一种新的、高度通用的方法来开发神经系统的准确模型。这些模型可以在三维中进行设计，同时选择组成模型的每个单独的组分，细胞和分子。模型的复杂程度可以根据需要从最简单的到更完整的进行调整。例如，通过使用脊髓的三维组织工程模型，有可能首次在体外再现运动神经元轴突周围髓鞘形成的过程。这一突破显示了组织工程在开发强大的神经系统体外模型方面的巨大潜力。这些模型与使用人成年神经元和神经胶质细胞的组合，这些神经元和神经胶质细胞是从患者的可接近组织（皮肤、脂肪、骨髓）中分离的神经前体细胞的分化中获得的，为更好地理解神经退行性疾病开辟了有希望的前景。
The nervous system is extraordinarily complex and exposed to various trauma and degenerative diseases that remain difficult to treat. To facilitate its study, in vitro models were developed by culturing neurons and glial cells in monolayer cultures, or through organotypic cultures of brain or spinal cord slices. These in vitro models were, and are still very helpful for the advancement of neurosciences. However, they are for some studies, either overly simplified, or too complex. The application of tissue engineering to neurosciences offers a new and highly versatile approach to develop accurate models of the nervous system. These models can be engineered in three-dimensions while choosing for each individual component, cellular and molecular, that will compose it. The level of complexity of the model can be adjusted from the simplest to the more complete as needed. For example, through the use of a three-dimensional tissue-engineered model of the spinal cord, it was possible to reproduce the process of myelin sheath formation around motor neuron axons for the first time in vitro. This breakthrough shows the promising potential of tissue engineering in the development of powerful in vitro models of the nervous system. The combination of these models with the use of human adult neurons and glial cells obtained from the differentiation of neural precursor cells isolated from accessible tissues from patients (skin, fat, bone marrow), opens promising perspectives to better understand -neurodegenerative diseases.
膜片钳记录“器官型”切片培养物中氨基酸激活的反应。
DOI：
10.1073/pnas.85.9.3221
发表时间：
1988
影响因子：
11.1
作者：
Llano,I;Marty,A;Johnson,JW;Ascher,P;Gähwiler,BH
通讯作者：
Gähwiler,BH
影响因子：
4.2
作者：
Geddes-Klein, DM;Schiffman, KB;Meaney, DF
通讯作者：
Meaney, DF