RODLETLESS, A NEW ASPERGILLUS DEVELOPMENTAL MUTANT INDUCED BY DIRECTED GENE INACTIVATION
RODLETLESS, A NEW ASPERGILLUS DEVELOPMENTAL MUTANT INDUCED BY DIRECTED GENE INACTIVATION
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DOI：
10.1101/gad.5.7.1161
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发表时间：
1991-07-01
影响因子：
10.5
通讯作者：
TIMBERLAKE, WE
中科院分区：
文献类型：
--
作者：
STRINGER, MA;DEAN, RA;TIMBERLAKE, WE
构巢曲霉 CAN41 转录单位在无性生殖器官（分生孢子梗）发育早期由 brlA 调节基因激活。 CAN41的破坏导致一种新的突变表型，其中分生孢子梗细胞和孢子缺乏外壁层，即小杆层，使得它们的疏水性比野生型低，导致孢子传播效率低下。无杆突变定义了 III 号染色体上的新基因座 rodA。 rodA 编码一种小的、中等疏水性的多肽，含有 8 个半胱氨酸，其排列模式与在裂褶全担子菌中观察到的三种疏水性细胞壁蛋白相似。我们提出，曲霉属和裂褶菌属 8-半胱氨酸多肽定义了一类分泌性疏水性真菌细胞壁蛋白，这些蛋白对于分生孢子梗和蘑菇等空中结构的形成和功能很重要。
The Aspergillus nidulans CAN41 transcription unit is activated by the brlA regulatory gene early during development of the asexual reproductive apparatus, the conidiophore. Disruption of CAN41 results in a novel mutant phenotype in which conidiophore cells and spores lack an external wall layer, the rodlet layer, making them less hydrophobic than in the wild type and leading to inefficient spore dispersal. The rodletless mutation defines a new locus on chromosome III, rodA. rodA encodes a small, moderately hydrophobic polypeptide containing 8 cysteines arranged in a pattern similar to that observed in three hydrophobic cell wall proteins from the Holobasidiomycete Schizophyllum commune. We propose that the Aspergillus and Schizophyllum 8-cysteine polypeptides define a class of secreted, hydrophobic, fungal cell wall proteins that are important in the formation and function of aerial structures such as conidiophores and mushrooms.