Performances of Lactobacillus brevis for Producing Lactic Acid from Hydrolysate of Lignocellulosics
Performances of Lactobacillus brevis for Producing Lactic Acid from Hydrolysate of Lignocellulosics
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DOI：
10.1007/s12010-009-8857-8
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发表时间：
2010-05-01
影响因子：
3
通讯作者：
Chen, Shulin
中科院分区：
文献类型：
--
作者：
Guo, Wei;Jia, Wendi;Chen, Shulin
利用从木质纤维生物质中提取的各种形式的糖，通过各种预处理和水解过程，是选择微生物生产生物燃料和生物化学品的主要标准。广泛的碳谱和潜在的抑制剂呋喃、苯酚化合物和弱酸是限制木质纤维素稀酸水解物在乳酸发酵中应用的两大障碍。从酸菜中分离到的两株细菌S3F4(短乳杆菌)和XS1T3-4(植物乳杆菌)能够利用生物质稀酸水解物中存在的各种糖类。S3F4菌株对潜在的发酵抑制剂阿魏酸和糠醛也表现出很强的抗性。该菌株摇瓶发酵产生的玉米芯稀酸水解液乳酸产量为39.1g/L，初始总糖浓度为56.9g/L(木糖46.4g/L；葡萄糖4.0g/L；阿拉伯糖6.5g/L)。玉米芯的水解液无需解毒即可被S3F4利用，且本研究得到的乳酸浓度高于其他报道。
Utilizing all forms of sugars derived from lignocellulosic biomass via various pretreatment and hydrolysis process is a primary criterion for selecting a microorganism to produce biofuels and biochemicals. A broad carbon spectra and potential inhibitors such as furan, phenol compounds and weak acids are two major obstacles that limited the application of dilute-acid hydrolysate of lignocellulosics in lactic acid fermentation. Two strains of bacteria isolated from sour cabbage, S3F4 (Lactobacillus brevis) and XS1T3-4 (Lactobacillus plantrum), exhibited the ability to utilize various sugars present in dilute-acid hydrolysate of biomass. The S3F4 strain also showed strong resistance to potential fermentation inhibitors such as ferulic acid and furfural. Fermentation in flasks by this strain resulted in 39.1 g/l of lactic acid from dilute acid hydrolysates of corncobs that had initial total sugar concentration of 56.9 g/l (xylose, 46.4 g/l; glucose, 4.0 g/l; arabinose, 6.5 g/l). The hydrolysate of corncobs was readily utilized by S3F4 without detoxification, and the lactic acid concentration obtained in this study was higher compared to other reports.