PREPARATION OF THERAPEUTIC PHOSPHOLIPIDS THROUGH PORCINE PANCREATIC PHOSPHOLIPASE A(2)-MEDIATED ESTERIFICATION AND LIPOZYME-MEDIATED ACIDOLYSIS
PREPARATION OF THERAPEUTIC PHOSPHOLIPIDS THROUGH PORCINE PANCREATIC PHOSPHOLIPASE A(2)-MEDIATED ESTERIFICATION AND LIPOZYME-MEDIATED ACIDOLYSIS
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DOI：
10.1007/bf02546201
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发表时间：
1995-11-01
影响因子：
2
通讯作者：
HATANO, M
中科院分区：
文献类型：
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作者：
HOSOKAWA, M;TAKAHASHI, K;HATANO, M
以大豆磷脂酰胆碱（PC）为原料，通过猪磷脂酶A（2）催化溶血磷脂酰胆碱酮的酯化反应和Lipozyme催化的酸解反应，制备了具有增强人红细胞变形能力的高不饱和脂肪酸磷脂（HUFA-PL）。通过这些过程，磷脂酶A（2），与甲酰胺作为水模拟物，促进HUFA掺入到PC的sn-2位，并抑制合成的PL的水解。在另一方面，脂肪酶介导的酸解之间的位置sn-1的大豆PC和HUFA的水和丙二醇的组合增强。同时，回收的PL产物显示新合成的健康有益的HUFA-PL的水解减少。
Highly unsaturated fatty acid-containing phospholipids (HUFA-PL), which serve to increase the deformability of human red blood cells, were prepared through porcine phospholipase A(2)-mediated esterification of the lysophosphatidylcholone, which was derived from soy phosphatidylcholine (PC), and by Lipozyme-mediated acidolysis. Through these processes, phospholipase A(2), with formamide as a water mimic, enhanced the incorporation of HUFA into position sn-2 of PC and suppressed hydrolysis of the synthesized PL. On the other hand, Lipozyme-mediated acidolysis between position sn-1 of soy PC and HUFA was enhanced by a combination of water and propylene glycol. Simultaneously, the recovered PL products showed decreased hydrolysis of newly synthesized health-beneficial HUFA-PL.